Membrane Protein Extraction Kit

SKU:BHT16300166
Overview
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Twelve non-denaturing extraction solutions for screening conditions that extract and stabilize membrane proteins from cell membranes. Kit has 0.5 ml of each solution; Kit Plus has 5 ml of each for larger preparative extractions.
Number of Conditions 12 extraction solutions
Pack Size 0.5 ml or 5 ml
Shipping Frozen
Options selector
Catalog no. Format
MPE01-12S Kit (12 solutions × 0.5 ml)
MPE01-12P Kit Plus (12 solutions × 5 ml)
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Format: Kit (12 solutions × 0.5 ml) – MPE01-12S; Kit Plus (12 solutions × 5 ml) – MPE01-12P
  • Difference between options: both formats contain the same 12 extraction solutions (#1–#12). Kit has 0.5 ml of each solution for screening and micro-scale preparative extraction (up to 1.5 mg membrane protein); Kit Plus has 5 ml of each for screening and middle-scale preparative extraction (up to 16 mg).
  • Next step: for large-scale extraction, order the best solution number as Membrane Protein Extraction Solution (MPE01 to MPE12, 40 ml each).
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: Please contact us to confirm storage conditions.
  • Shipping: Shipped frozen.
  • Upon receipt: unpack promptly.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Applications
  • Protein Extraction
Storage Please contact us to confirm storage conditions.
Shipping Shipped frozen.
Catalog no. (Mfr.) MPE01-12S | MPE01-12P
Main SKU BHT16300166
Membrane Protein Extraction

Product Overview

The Membrane Protein Extraction Kit provides 12 extraction solutions for extracting and stabilizing membrane proteins from cell membranes. The solutions are based on different non-denaturing detergents and synthetic lipid analogs: alkyl saccharides (#1, #2), alkylpolyethylenes (#3, #5, #11), acyl-N-methylglucamide (#4), a bile acid salt (#6), an alkylaminoxide (#7), zwitterionic detergents (#8, #9, #12) and a synthetic phosphocholine derivative (#10).

Screening the 12 solutions side by side shows which condition extracts your membrane protein best. The selected solution is also available on its own in a 40 ml size for large-scale extraction (Membrane Protein Extraction Solution).

Product Description

Option Kit Kit Plus
Catalog # MPE01-12S MPE01-12P
Solutions 12 (#1–#12) 12 (#1–#12)
Volume of each solution 0.5 ml 5 ml
Use Screening and micro-scale preparative extraction Screening and middle-scale preparative extraction
Membrane protein per kit Up to 1.5 mg Up to 16 mg

Specifications

Format 12 extraction solutions
Extraction agents Non-denaturing detergents and synthetic lipid analogs
Catalog # MPE01-12S, MPE01-12P
Storage Please contact us to confirm storage conditions.
Shipping Shipped frozen.

Protocol Outline

  1. Prepare cell membranes (for example by high-speed centrifugation of the cell lysate), and place membranes containing about 0.2–0.3 mg of membrane protein in 12 tubes.
  2. Pellet the membranes at 100,000 x g for 45 min and remove the supernatant.
  3. Add 100 µl of each extraction solution (with protease inhibitors) to a different tube, suspend the membranes well and incubate.
  4. Separate the extracted proteins from the insoluble material and compare the extraction efficiency of the 12 solutions.

The ProFoldin protocol “Membrane Protein Extraction” is linked under Documents.

Notes

  • Catalog #s MPE01-12S (Kit) and MPE01-12P (Kit Plus) contain all 12 solutions for screening. Catalog #s MPE01 to MPE12 are single 40 ml bottles of one solution (Membrane Protein Extraction Solution).

Safety & Handling

For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.
Q.What is the difference between Kit and Kit Plus?
A.Both contain the same 12 extraction solutions (#1–#12). Kit (MPE01-12S) has 0.5 ml of each solution, for up to 1.5 mg membrane protein; Kit Plus (MPE01-12P) has 5 ml of each, for up to 16 mg.
Q.What kinds of extraction agents are included?
A.Non-denaturing detergents and synthetic lipid analogs: alkyl saccharides, alkylpolyethylenes, acyl-N-methylglucamide, a bile acid salt, an alkylaminoxide, zwitterionic detergents and a synthetic phosphocholine derivative.
Q.Can I scale up with the best solution?
A.Yes. Each solution is also sold on its own in a 40 ml size (Membrane Protein Extraction Solution) for large-scale extraction.
Q.Is MPE01-12S the same as MPE01 to MPE12?
A.No. MPE01-12S (Kit) and MPE01-12P (Kit Plus) contain all 12 solutions for screening. MPE01 to MPE12 are single 40 ml bottles of one selected solution, sold as Membrane Protein Extraction Solution.
Q.Is a protocol available?
A.Yes. The ProFoldin protocol “Membrane Protein Extraction” is linked under Documents.
Q.How is it shipped and stored?
A.Shipped frozen. Please contact us to confirm storage conditions.

Can’t find the exact format you need? We can help you source or customize this Membrane Protein Extraction Kit — including larger pack sizes or bulk orders. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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