| Field | Specification |
|---|---|
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Product Overview
The Membrane Protein Extraction Kit provides 12 extraction solutions for extracting and stabilizing membrane proteins from cell membranes. The solutions are based on different non-denaturing detergents and synthetic lipid analogs: alkyl saccharides (#1, #2), alkylpolyethylenes (#3, #5, #11), acyl-N-methylglucamide (#4), a bile acid salt (#6), an alkylaminoxide (#7), zwitterionic detergents (#8, #9, #12) and a synthetic phosphocholine derivative (#10).
Screening the 12 solutions side by side shows which condition extracts your membrane protein best. The selected solution is also available on its own in a 40 ml size for large-scale extraction (Membrane Protein Extraction Solution).
Product Description
| Option | Kit | Kit Plus |
|---|---|---|
| Catalog # | MPE01-12S | MPE01-12P |
| Solutions | 12 (#1–#12) | 12 (#1–#12) |
| Volume of each solution | 0.5 ml | 5 ml |
| Use | Screening and micro-scale preparative extraction | Screening and middle-scale preparative extraction |
| Membrane protein per kit | Up to 1.5 mg | Up to 16 mg |
Specifications
| Format | 12 extraction solutions |
|---|---|
| Extraction agents | Non-denaturing detergents and synthetic lipid analogs |
| Catalog # | MPE01-12S, MPE01-12P |
| Storage | Please contact us to confirm storage conditions. |
| Shipping | Shipped frozen. |
Protocol Outline
- Prepare cell membranes (for example by high-speed centrifugation of the cell lysate), and place membranes containing about 0.2–0.3 mg of membrane protein in 12 tubes.
- Pellet the membranes at 100,000 x g for 45 min and remove the supernatant.
- Add 100 µl of each extraction solution (with protease inhibitors) to a different tube, suspend the membranes well and incubate.
- Separate the extracted proteins from the insoluble material and compare the extraction efficiency of the 12 solutions.
The ProFoldin protocol “Membrane Protein Extraction” is linked under Documents.
Notes
- Catalog #s MPE01-12S (Kit) and MPE01-12P (Kit Plus) contain all 12 solutions for screening. Catalog #s MPE01 to MPE12 are single 40 ml bottles of one solution (Membrane Protein Extraction Solution).
Safety & Handling
Can’t find the exact format you need? We can help you source or customize this Membrane Protein Extraction Kit — including larger pack sizes or bulk orders. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.