| Field | Specification |
|---|---|
| Target | |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C22H23ClN4 |
| Purity | |
| SMILES | |
| Form | Powder |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
Methylene Violet 3RAX is a phenazine dye used to stain mitochondria in cells. It can alter the molecular structure of DNA, disrupt DNA modules, and induce the generation of reactive singlet oxygen. The dye inhibits human erythrocyte AChE and human plasma BChE with Ki values of 1.58 μM and 0.51 μM, respectively, and has potential as a photosensitizer for mitochondrial-targeted action in photodynamic therapy (PDT)[1][2]. It is supplied as a green to dark green powder (C22H23ClN4, MW 378.90) at 95.54% purity.
Physical & Chemical Properties
| CAS Number | 4569-86-2 |
|---|---|
| Molecular Formula | C22H23ClN4 |
| Molecular Weight | 378.90 g/mol |
| Purity | 95.54% |
| Appearance | Powder |
| Color | Green to dark green |
| SMILES | [Cl-].N=1C=2C=CC(N)=CC2[N+](C=3C=CC=CC3)=C4C=C(C=CC14)N(CC)CC |
| Target | BChE, AChE |
| Signaling Pathway | Neuronal Signaling |
| Solubility | In Vitro: DMSO: 25 mg/mL (65.98 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | 4°C, protect from light. In solvent: -80°C, 6 months; -20°C, 1 month (protect from light). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Biological Activity
IC50 & Target[2]
|
BChE 0.51 μM (Ki) |
AChE 1.58 μM (Ki) |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 25 mg/mL (65.98 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); protect from light; avoid repeated freeze-thaw cycles.
Data provided by the manufacturer.
In Vitro
Methylene Violet 3RAX displays antitumor activity by cleaving DNA strands in tumor cells[1]. Methylene Violet 3RAX (0-400 μM) markedly enhances DNA cleavage, with over 60% photocleavage activity toward DNA at 300 μM[1].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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