| Field | Specification |
|---|---|
| Target | |
| Alternative names | Methylated-DNA--protein-cysteine methyltransferase|6-O-methylguanine-DNA methyltransferase (MGMT)|O-6-methylguanine-DNA-alkyltransferase|MGMT |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | O-6-methylguanine-DNA methyltransferase |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Involved in the cellular defense against the biological effects of O6-methylguanine(O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes MGMT. It is validated for ELISA, Western Blot, Immunohistochemistry.
Validated Applications
| Applications | ELISA, Western Blot, Immunohistochemistry |
|---|---|
| WB | 1:500-1:5000 |
| IP | 1:200-1:2000 |
| IHC | 1:20-1:200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
O-6-methylguanine-DNA methyltransferase
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Immunogen | O-6-methylguanine-DNA methyltransferase |
| Purification | Immunogen Affinity Purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Form | Liquid |
| Target MW | 22 kDa |
| UniProt # | P16455 |
| Gene ID | 4255 |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Alternative Names | Methylated-DNA--protein-cysteine methyltransferase|6-O-methylguanine-DNA methyltransferase (MGMT)|O-6-methylguanine-DNA-alkyltransferase|MGMT |
Reactivity Notes
Validated reactivity: Human. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for MGMT research from Fine Test, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
