| Field | Specification |
|---|---|
| Target | |
| Alternative names | E3 ubiquitin-protein ligase mib1 (EC 2.3.2.27) (Protein mind bomb) (RING-type E3 ubiquitin transferase mib1), mib1, mib |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Zebrafish E3 ubiquitin-protein ligase mib1 protein (1-274AA) |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This polyclonal anti-mib1 antibody raised in Rabbit, conjugated with HRP, tested in ELISA. Designed to detect mib1 in Zebrafish for others research.
Key elements and design rationale
- Immunogen: Recombinant Zebrafish E3 ubiquitin-protein ligase mib1 protein (1-274AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
- HRP: Direct detection (TMB/ECL); quench endogenous peroxidase in tissue assays.
Biological background
mib1 (also: E3 ubiquitin-protein ligase mib1 (EC 2.3.2.27) (Protein mind bomb) (RING-type E3 ubiquitin transferase mib1), mib1, mib) — studied in Danio rerio (Zebrafish) (Brachydanio rerio) systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current mib1 annotation relevant to others.
Common research applications
- ELISA: Quantify soluble target; ensure samples within the linear detection range.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Zebrafish; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
mib1 is a protein studied in Zebrafish biology. This polyclonal antibody raised in Rabbit detects mib1 in ELISA applications, IgG isotype.
Reported reactive against Zebrafish. Immunogen from Danio rerio (Zebrafish) (Brachydanio rerio). Cross-reactivity with untested species requires empirical confirmation.
Reported for ELISA. Optimize dilution/blocking per application. Consult datasheet for working concentrations.
HRP: direct detection (TMB/ECL); quench endogenous peroxidase in tissue assays.
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing mib1; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
