| Field | Specification |
|---|---|
| Alternative Names | MIB2; SKD; ZZANK1; E3 ubiquitin-protein ligase MIB2; EC 2.3.2.27; Mind bomb homolog 2; Novel zinc finger protein; Novelzin; Putative NF-kappa-B-activating protein 002N; RING-type E3 ubiquitin transferase MIB2; Skeletrophin; Zinc finger ZZ type with ankyrin repeat domain protein 1 |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Human E3 ubiquitin-protein ligase MIB2 protein (1-128AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a polyclonal anti-MIB2 antibody raised in Rabbit, conjugated with HRP, with confirmed utility in ELISA. It is designed to detect MIB2 protein in Human and supports researchers working in signal transduction contexts.
Key elements and design rationale
- Immunogen: Recombinant Human E3 ubiquitin-protein ligase MIB2 protein (1-128AA) — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
- Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
- Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Protein G purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
- HRP conjugate: Direct detection with TMB/OPD or ECL substrates. Quench endogenous peroxidase before use in tissue assays.
Biological background
MIB2 (also referred to as MIB2, SKD, ZZANK1, E3 ubiquitin-protein ligase MIB2, EC 2.3.2.27, Mind bomb homolog 2, Novel zinc finger protein, Novelzin, Putative NF-kappa-B-activating protein 002N, RING-type E3 ubiquitin transferase MIB2, Skeletrophin, Zinc finger ZZ type with ankyrin repeat domain protein 1) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of MIB2 biology in signal transduction.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
MIB2 is a protein target in Human biology. This polyclonal antibody raised in Rabbit is designed to detect MIB2 in ELISA applications, with IgG isotype.
Reported reactive against Human. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
HRP enables direct TMB/ECL detection without secondary. Quench endogenous peroxidase in tissue assays.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — known MIB2-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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