| Field | Specification |
|---|---|
| Alternative Names | CasL interacting molecule; DKFZp434B1517; FLJ11937; FLJ21739; MICA1_HUMAN; MICAL 1; MICAL; MICAL1; microtubule associated monoxygenase; calponin and LIM domain containing 1; Molecule interacting with CasL protein 1; NEDD9 interacting protein with calponin homology and LIM domains; NEDD9-interacting protein with calponin homology and LIM domains; NICAL; OTTHUMP00000016969 |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Human [F-actin]-methionine sulfoxide oxidase MICAL1 protein (806-1067AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a polyclonal anti-MICAL1 antibody raised in Rabbit, conjugated with Biotin, with confirmed utility in ELISA. It is designed to detect MICAL1 protein in Human and supports researchers working in tags & cell markers contexts.
Key elements and design rationale
- Immunogen: Recombinant Human [F-actin]-methionine sulfoxide oxidase MICAL1 protein (806-1067AA) — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
- Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
- Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Protein G purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
- Biotin conjugate: Amplified detection via streptavidin/avidin reporters. Block endogenous biotin in biotin-rich tissue samples.
Biological background
MICAL1 (also referred to as CasL interacting molecule, DKFZp434B1517, FLJ11937, FLJ21739, MICA1_HUMAN, MICAL 1, MICAL, MICAL1, microtubule associated monoxygenase, calponin and LIM domain containing 1, Molecule interacting with CasL protein 1, NEDD9 interacting protein with calponin homology and LIM domains, NEDD9-interacting protein with calponin homology and LIM domains, NICAL, OTTHUMP00000016969) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of MICAL1 biology in tags & cell markers.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
MICAL1 is a protein target in Human biology. This polyclonal antibody raised in Rabbit is designed to detect MICAL1 in ELISA applications, with IgG isotype.
Reported reactive against Human. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
Biotin enables streptavidin/avidin-amplified detection. Block endogenous biotin in biotin-rich tissues.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — known MICAL1-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Research budgets are tight — we get it. That's why we've put together a fresh round of exclusive promotions designed to help you stock up on the reagents, kits, and consumables your lab depends on, without stretching your budget.
🔬 What's on offer right now:
10% Off Pre-Designed siRNA Sets
20% Off Transmembrane Proteins
50% Off Lab Consumables + Free Shipping
$99 Pipette Filler Promotion Package
BlasTaq 2X qPCR MasterMix - 50% OFF Limited Time Offer
DENARASE® Endonuclease — 10% Off One Order
