Micro Phosphate Removal Column Set

What it means: Spin Column — the column format used to process each sample.

Why it matters:

• Spin columns need only a bench-top microcentrifuge and 1.5 ml tubes as receivers.
• Each column processes one sample, so several samples can be run in parallel in the same rotor.
• Separation takes a few short spins instead of a gravity column run.
• Pre-packed columns avoid packing resin by hand.
• The eluate is collected directly in the receiver tube; a final spin or 0.22 µm filtration removes any insoluble material.

For this product: Each spin column is pre-packed and ready to use after the preparation spins described in the ProFoldin protocol.

Tip: Cut the caps off the receiver tubes before use, as the protocol describes, so the column sits correctly during spins.

What it means: 20 columns — how many columns come in one pack.

Why it matters:

• Each column processes one 200 µl sample load, so one pack covers up to 20 samples.
• Larger sample volumes use more columns, or repeated loads on the same column where the protocol allows it.
• Including control samples, such as a buffer-only load, uses columns from the same pack.
• Columns are used one at a time, so unused columns can stay in the pack until needed.
• Running a whole sample series from one pack keeps the column preparation the same for every sample.

For this product: Each pack of this product contains 20 columns, each pre-packed and ready for the preparation spins.

Tip: Count samples, repeats and controls before starting so that one pack covers the whole experiment.

What it means: 200 µl — the sample volume loaded onto one column per spin.

Why it matters:

• Load 200 µl of sample per column after the resin has been spun almost dry.
• Larger volumes are processed by repeating the load until the phosphate binding capacity of the resin is reached.
• Staying within the stated volume keeps separation performance as described in the protocol.
• Loading the same volume on every column makes recovery easy to compare between samples.
• Have samples ready before starting the column preparation spins.

For this product: Load 200 µl per column. Spin speeds and times are given in the ProFoldin protocol under Documents.

Tip: Measure 200 µl carefully for each load, and use the same volume for every column in a comparison.

What it means: 200 µmol phosphate/ml bed volume — how much phosphate the column resin can hold.

Why it matters:

• Phosphate binds to the resin and stays on the column, while buffer salts, sugars and glycerol stay in the sample.
• ATP and some other organic phosphates also bind, but less strongly than inorganic phosphate.
• Metal chelators such as EDTA interfere with phosphate binding to the resin.
• Repeated loads on the same column are possible until the phosphate capacity of the resin is reached.

For this product: The resin binds 200 µmol phosphate/ml bed volume. Per column, a 0.2 ml sample is reduced from 500 mM to 3 mM phosphate, or from 10 mM to 0.0003 mM.

Tip: Repeat the load on a fresh column if needed.

What it means: Cold, Not Frozen — how the product is shipped.

Why it matters:

• The shipping condition tells you what to expect when the package arrives.
• Unpacking promptly lets you check the contents against the order.
• Checking the package on arrival helps you spot transport problems early.
• Keeping the shipping box until the contents are checked makes any claim easier.
• Storage conditions can differ from shipping conditions, so confirm them before the package arrives.
• Recording the arrival date and condition helps if questions come up later.

For this product: Cold, Not Frozen: Shipped cold (not frozen). Please contact us for storage conditions.

Tip: Open and check the package on arrival, and contact us right away if anything is damaged or missing.