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| Assay area | |
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| Main SKU |
Kinases transfer a phosphate from ATP to a substrate, giving a phosphorylated substrate and ADP. Measuring the ADP formed is a general way to follow the activity of purified kinases regardless of the substrate.
Pack Sizes
Both sizes contain the same reagents. The only difference is the number of assays: MUK100K is for 100 assays and MUK1000K for 1000 assays (384-well format); see the Kit Components table for the volumes of each size.
| 100 assays (MUK100K) | 100 assays; enzyme not included |
|---|---|
| 1000 assays (MUK1000K) | 1000 assays; enzyme not included |
Kit Components
| Component | 100 assays (MUK100K) | 1000 assays (MUK1000K) |
|---|---|---|
| 10 x Assay buffer | 600 µl | 4000 µl |
| 100 x ATP | 35 µl | 350 µl |
| 100 x MUK Reagent A | 35 µl | 350 µl |
| 100 x MUK Reagent B | 35 µl | 350 µl |
| 1 mM ADP | 30 µl | 200 µl |
| 10 x Fluorescence dye | 350 µl | 3500 µl |
The 1 x MUK Buffer is 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 3 mM MgCl2, 0.5 mM DTT, 0.2 mM EDTA, 0.003% Brij-35. Kinase and kinase substrate are supplied by the user.
Assay Principle
The kit measures the ADP produced by the kinase reaction. MUK Reagents A and B are added after a 2 min pre-incubation and are present during the 60 min reaction; a fluorescence dye is then added to read the ADP signal at 535 nm (excitation 485 nm). Low ATP (20 µM recommended) keeps the ADP background low and lets ATP-competitive inhibitors be detected.
Specifications
| Assay Target | Kinase activity (ADP formation) |
|---|---|
| Target Type | Kinase |
| Assay Type | Enzyme Activity Assay; Inhibitor Screening Assay |
| Detection Method | Fluorescence |
| Detection Wavelength | Ex 485 nm / Em 535 nm |
| Plate Format | 384/96-well plate or micro-cuvette |
| Instrument Required | Plate reader or fluorometer |
| Assay Time | About 62 min (2 min pre-incubation + 60 min reaction) |
| Measuring Range | 0.1 µM to 10 µM ADP |
| Recommended ATP | 20 µM |
| Sizes | 100 assays, 1000 assays |
| Storage | Please contact us to confirm storage conditions. |
| Shipping | Shipped frozen. |
Protocol
The manufacturer's protocol describes the procedure and reaction volumes.
- Universal Kinase Assay (PDF, see Documents): Covers catalog # MUK100K, MUK1000K.
Safety & Handling
Can’t find the exact format you need? We can help you source or customize this kinase activity (ADP formation) assay — including larger pack sizes, bulk reagents, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.
