| Field | Specification |
|---|---|
| Mfr No | |
| Form | Kit |
| Product Type | |
| Storage |
| Product Component | Quantity |
|---|---|
| 2X miRNA cDNA Synthesis SuperMix | 20 rxn (200 μl) |
| Enzyme Mix | 20 rxn (40 μl) |
| Nuclease-Free H2O | 1.0 ml |
| Component | Quantity |
|---|---|
| 2X miRNA cDNA Synthesis SuperMix | 20 rxn (200 μl) |
| Enzyme Mix | 20 rxn (40 μl) |
| Nuclease-Free H2O | 1.0 ml |
| Specification | Value |
|---|---|
| Enzyme Type | cDNA Synthesis Kit |
| Format | Kit |
| Storage Conditions | Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly. |
Yes, the miRNA All-In-One cDNA Synthesis Kit is designed for synthesizing cDNA from miRNAs, which can be directly used for qPCR-based analysis. Pair with BlasTaq™ qPCR MasterMix (sold separately, Cat. No. G891) and the appropriate miRNA forward primer from our miRNA qPCR Primer Library.
Both small RNA (200 ng) and total RNA (up to 2 μg) can be used. Small RNA typically yields better results, but both types are compatible with the kit.
The kit includes RNaseOFF Ribonuclease Inhibitor, which protects RNA from degradation during the cDNA synthesis process.
The modified Oligo d(T) with Adapter binds initiates polyA-tailing and reverse transcription of the mature miRNAs enabling the synthesis of cDNA for subsequent analysis. The adapter contains the Universal reverse sequence enabling easy quantification through real-time PCR.
The kit is optimized for use with our miRNA primers, but other primers compatible with the Universal 3' miRNA Reverse Primer may also work. For optimal results, we recommend using our forward miRNA primers found in the miRNA qPCR Primer Library.
Yes, we offer custom primer design services for miRNAs not currently available in our catalog. Contact [contact ABM technical support] with the miRNA accession numbers, and we'll be happy to design the primers for you.
The synthesized miRNA cDNA can be stored at -20°C for long-term use without compromising its quality.
Yes, we offer various housekeeping gene primers, including U6 (Cat. No. MPH00001), SNORD44 (Cat. No. MPH00003), and SNORD48 (Cat. No. MPH00005), which can be used as internal controls in qPCR.
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