| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C21H17N5O2S |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
MKA031 is a non-competitive inhibitor of MIF, with an IC50 of 1.7 μM. It interferes with the MIF/AIF interaction, MIF nuclear translocation, and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced cell death, and it can be used to study chronic hepatitis C virus infection[1]. It is supplied as an off-white to light yellow solid (C21H17N5O2S, MW 403.46) at 98.69% purity.
Physical & Chemical Properties
| CAS Number | 2951012-00-1 |
|---|---|
| Molecular Formula | C21H17N5O2S |
| Molecular Weight | 403.46 g/mol |
| Purity | 98.69% |
| Appearance | Solid |
| Color | Off-white to light yellow |
| SMILES | O=C(NC1=CC=CC=C1)C2=CC=C(C=C2)N3N=NC(C(NCC4=CC=CS4)=O)=C3 |
| Signaling Pathway | Immunology/Inflammation |
| Solubility | In Vitro: DMSO: 25 mg/mL (61.96 mM; Requires sonication and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Powder: -20°C, 3 years. In solvent: -80°C, 6 months; -20°C, 1 month. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 25 mg/mL (61.96 mM) | requires sonication and warming and heat to 60°C; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); avoid repeated freeze-thaw cycles.
Data provided by the manufacturer.
In Vitro
MKA031 (1-100 μl; 3 h) protects against MNNG-induced cell death[1]. MKA031 (100 μl;3 h) protects genomic DNA by blocking MIF recruitment to AIF[1].
Cell Viability Assay[1]
| Cell Line | Hela cells |
|---|---|
| Concentration | 1-100 μM |
| Incubation Time | 3 h |
| Result | Protected HeLa cells from MNNG-induced parthanatos in a dose dependent manner with an EC50 of 7.7 μM. |
Immunofluorescence[1]
| Cell Line | Hela cells |
|---|---|
| Concentration | 100 μM |
| Incubation Time | 3 h |
| Result | Interfered with the MIF/AIF interaction and blocks MIF nuclear translocation. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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