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The MKN-7 cell line is a well-characterized human gastric carcinoma cell line, established from a well-differentiated tubular adenocarcinoma. This cell line is part of a broader panel of gastric cancer cell lines that were developed to study the varied histological and biological behaviors of gastric carcinomas. MKN-7 cells are known to display morphological characteristics indicative of intestinal differentiation, such as cell polarity and the presence of microvilli with core filaments. These features are typically observed in both in vitro cultures and in xenografts in nude mice, although the degree of differentiation may diminish over time with prolonged culture conditions. In terms of functional characteristics, MKN-7 cells exhibit low fibrinolytic activity, which is primarily plasminogen-dependent. This activity is significantly lower compared to other gastric cancer cell lines like MKN-1 and MKN-28, which show higher fibrinolytic activities. The low fibrinolytic activity of MKN-7 cells may be relevant in studies investigating the role of fibrinolysis in cancer progression, particularly in relation to the invasive and metastatic potential of gastric tumors. Furthermore, the MKN-7 cell line, along with other gastric cancer cell lines, has been utilized in studies examining thromboplastic activity, though MKN-7 is noted for its relatively low levels of this activity as well. This suggests a more limited role in the hypercoagulable states often associated with aggressive tumor phenotypes.
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- cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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