| Field | Specification |
|---|---|
| Mfr No | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
MLL3/WARD complex of human MLL3 (GenBank Accession No. NM_170606) a.a. 4689-4911(end) with N-terminal His-tag and MW= 27 kDa, human WDR5 (GenBank Accession No. NM_017588), a.a. 2-334(end ) with N-terminal His-tag and MW= 37 kDa, human Ash2L (GenBank Accession No. NM_001105214), a.a. 2-534(end) with N-terminal His-tag and MW= 61 kDa, human RbBP5 (GenBank Accession No. NM_005057), a.a. 2-538(end) with N-terminal His-tag and MW= 60 kDa, and human DPY30 (GenBank Accession No. NM_032574) a.a. 2-99(end) with N-terminal His-tag and MW=12 kDa individually expressed in an E. coli expression system.
Product Description
Recombinant ProteinRecombinant MLL3/WDR5/Ash2L/RbBP5/DPY30 Complex Human protein is produced using a validated recombinant expression system and supplied in aqueous buffer solution. Suitable for enzyme kinetics, inhibitor screening, binding assays, structural studies, and related biochemical research applications.
Protein Specifications
| UniProt ID | MLL3: Q8NEZ4; WDR5: P61964; Ash2L: Q9UBL3; RbBP5: Q15291; DPY30: Q9C005 |
|---|---|
| Amino Acids / Region | MLL3 4689-endother components 2-end |
| Affinity Tag | N-terminal His-tag (all) |
| Molecular Weight | 183 kDa (complex) |
| Formulation | 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 20… |
| Storage | At least 6 months at -80°C. |
| Biosafety Level | Not applicable (BSL-1) |
Safety & Handling
FOR RESEARCH USE ONLY (RUO). Not for diagnostic or therapeutic applications.
This protein was produced in a recombinant expression system. Expression system selection determines post-translational processing, disulfide bond formation, and co-factor incorporation — all of which affect enzymatic activity. Insect cell (Sf9) systems are preferred for kinases and multi-subunit enzymes that require phosphorylation or chaperone assistance; E. coli is used for structurally simpler proteins.
This protein spans amino acids MLL3 4689-endother components 2-end. Confirm the region includes your domain of interest — the active site, binding pocket, or substrate recognition sequence — before placing your order. Refer to the UniProt database for domain annotation.
Purity is assessed by SDS-PAGE; see the Certificate of Analysis. A gel image is provided with each lot. BPS Bioscience performs rigorous QC on each lot, including purity assessment and functional activity testing where applicable. Contact technical support if purity ≥99% is required for biophysical measurements.
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Refer to the product datasheet for validated protocols and recommended assay conditions. Contact BioHippo technical support for application-specific guidance.
At least 6 months at -80°C. Avoid repeated freeze-thaw cycles — prepare single-use working aliquots. Add BSA or glycerol to aliquots if storing diluted enzyme is necessary. Typical stability is at least 6 months at −80°C.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Bulk quantities: Large-scale orders for HTS campaigns or structural studies.
- Custom constructs: Alternative tag positions, truncation variants, or point mutants may be available upon request.
- Biotinylated variants: Avi-Tag site-specific biotinylation is available for SPR/BLI surface capture applications.
- Extended QC data: Activity assay data, SEC-HPLC profiles, or additional purity methods available on request.
Contact BioHippo customer support for custom requirements.
- Patel, A., et al., J. Biol. Chem. 2009; 284(36): 24242-24256.
- Mohan, M., et al., Nat. Rev. Cancer 2010; 10(10): 721-728.
