| Field | Specification |
|---|---|
| Mfr No | |
| Form | Liquid |
| Product Type | |
| Relative Density | |
| Shipping | |
| Storage |
Product Overview
MonoMag Carboxyl Beads Conjugation Kit is a Conjugation Kit from Ocean NanoTech.
Ocean NanoTech’s carboxyl terminated Mono Mag magnetic beads of various diameters (1 µm, 3 µm and 4.5 µm) are now available in an easy-to-use kit format to enable researchers to conjugate antibodies, proteins, peptides or other biomolecules of their own choice to these magnetic beads. Attribute to the large surface area, uniform size, narrow size distribution and unique surface coating of the MonoMag Carboxyl Beads as well as the specifically formulated conjugation buffers, the resulting conjugates exhibit high binding capacity and low non-specific binding of protein or nucleic acids. The kit contains sufficient reagents & components for performing at least 5 conjugation reactions. All componets in the kit have been autoclaved. Briefly, the magnetic beads are activated using carbodiimide and N-hydroxysuccinimide followed by conjugation to amino groups that are present on the target protein/ligand. The recommended protocol has been used to successfully conjugate bovine serum albumin, streptavidin or immunoglobulin to these magnetic beads.
Kit Contents
- MonoMag Carboxyl Beads: 2 mL (10 mg/mL)
- Activation Buffer: 15 mL
- Quenching Buffer: 1 mL
- Storage Buffer: 30 mL
- EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide), 20 mg (Store at -20 °C upon arrival)
- Sulfo-NHS: 20 mg (Store at -20 °C upon arrival)
Physical & Chemical Properties
| pH | 6.0 - 8.0 at 25 °C (77 °F) |
|---|---|
| Solubility | Completely miscible |
| Chemical Stability | Stable under recommended storage conditions. |
| Incompatible Materials | Strong oxidizing agents |
Safety & Handling
For Research Use Only (RUO). Consult the Safety Data Sheet (SDS) before use and follow all applicable safety protocols.
This conjugation kit is optimized for covalent attachment of proteins, antibodies, peptides, and other amine- or carboxyl-containing biomolecules to the nanoparticle surface. The conjugation chemistry exploits EDC/NHS carbodiimide coupling or direct amine coupling depending on the specific kit protocol. Refer to the product data sheet for the list of validated biomolecule types and loading ratios.
This kit is available in multiple particle size variants (accessible via the variant selector). Particle size affects hydrodynamic behavior, conjugation efficiency, and downstream assay sensitivity. Smaller particles (10–15 nm) are preferred for solution-phase immunoassays, while larger particles (20–30 nm) may be better suited for lateral flow or magnetic separation applications.
Conjugation efficiency can be assessed by measuring protein concentration in the pre- and post-conjugation supernatant using a BCA or Bradford assay to estimate the amount of protein immobilized on the nanoparticle surface. Gel electrophoresis, DLS (dynamic light scattering), and functional assays (e.g., antibody binding activity) are recommended for comprehensive conjugate characterization.
Conjugated nanoparticles should be stored in the supplied storage buffer at 2–8°C and protected from light. Avoid freezing, as freeze-thaw cycles can cause irreversible aggregation. Short-term storage at room temperature is acceptable for applications requiring immediate use, but long-term stability should be evaluated under intended storage conditions.
BioHippo partners with leading life science suppliers to offer flexible purchasing and support options. Contact our team to discuss the following add-on services.
- Bulk & Volume Pricing: Reduced per-unit pricing for multi-unit or project-scale orders. Submit a quote request for a customized offer.
- Expedited Fulfillment: Priority order handling may be available for time-sensitive projects. Confirm availability before ordering.
- Technical Consultation: Our scientific team provides protocol guidance and troubleshooting at no additional charge.
- Custom Configurations: Alternative formulations, pack sizes, or kit configurations may be available upon request.
Use the contact form or live chat on ebiohippo.com to connect with a product specialist.
