| Field | Specification |
|---|---|
| Target | |
| Alternative names | Inhibin beta A chain;Activin beta-A chain;Inhba; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: CHO; Immunogen sequence: G311-S426 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma, Saliva |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse inhibin beta A chain (gene Inhba) in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Inhibin beta A (INHBA) forms the homodimer activin A and, with the alpha subunit, inhibin A. Activin A is a TGF-β family ligand that signals through type I and type II activin receptors to phosphorylate SMAD2 and SMAD3, regulating embryonic development, stem cell differentiation, reproduction, inflammation and tissue fibrosis.
Kit Components
| Anti-Mouse Inhba Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse Inhba Standard | 2 vials, 10 ng/tube |
| Mouse Inhba Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, serum, plasma (heparin, EDTA) and saliva |
| Assay range | 15.6 pg/mL–1,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant mouse Inhba (Gly311–Ser426), expressed in CHO |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 5.6–7.6% (3 samples, n = 16 each) |
| Inter-assay CV | 5.6–7.9% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured 62–422 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.025; 15.6 = 0.074; 31.2 = 0.121; 62.5 = 0.2; 125 = 0.387; 250 = 0.683; 500 = 1.251; 1000 = 1.916. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.025 | 0.074 | 0.121 | 0.2 | 0.387 | 0.683 | 1.251 | 1.916 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 40 | 155 | 440 | 38 | 146 | 460 |
| Standard deviation | 3.04 | 9.92 | 24.64 | 2.24 | 11.53 | 25.76 |
| CV (%) | 7.6% | 6.4% | 5.6% | 5.9% | 7.9% | 5.6% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Eri Ueno et al. (2025) 17β-estradiol and estrogen receptor alpha protect mouse ovarian follicle development by repressing atresia. iScience. 10.1016/j.isci.2025.111846
Shuxian Cai et al. (2018) Inhibition by microbial metabolites of Chinese dark tea of age-related neurodegenerative disorders in senescence-accelerated mouse prone 8 (SAMP8) mice. Food & Function. 10.1039/C8FO01512K
Hongjun Zhang et al. (2016) Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling. Basic & Clinical Pharmacology & Toxicology. 10.1111/bcpt.12749
Dieelberg Catharina et al. (2012) Follistatin Does Not Influence the Course of Escherichia coli K1 Sepsis in a Mouse Model. Shock. 10.1097/SHK.0b013e3182748d96
Hongjun Zhang et al. (2018) Adenovirus‑mediated knockdown of activin A receptor type 2A attenuates immune‑induced hepatic fibrosis in mice and inhibits interleukin‑17‑induced activation of primary hepatic stellate cells. International Journal of Molecular Medicine. 10.3892/ijmm.2018.3600
Lianli He et al. (2020) Ginsenoside Rg1 improves pathological damages by activating the p21‑p53‑STK pathway in ovary and Bax‑Bcl2 in the uterus in premature ovarian insufficiency mouse models. Molecular Medicine Reports. 10.3892/mmr.2020.11675
Xiaozhen Liu et al. (2014) Effects of nonylphenol exposure on expression of cell receptors and secretory function in mouse Sertoli TM4 cells. Environmental Toxicology and Pharmacology. 10.1016/j.etap.2014.01.017
Yu Wu et al. (2018) Biological effects of autophagy in mice with sepsis‑induced acute kidney injury. Experimental and Therapeutic Medicine. 10.3892/etm.2018.6899
