| Field | Specification |
|---|---|
| Target | |
| Alternative names | Angiopoietin-1;ANG-1;Angpt1;Agpt; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: H16-F497 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse angiopoietin-1 (gene Angpt1) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Angiopoietin-1 (ANGPT1) is a secreted ligand that activates the endothelial receptor tyrosine kinase TIE2 (TEK). TIE2 signaling from ANGPT1 promotes endothelial survival and vessel maturation and keeps mature vessels stable and less leaky, so ANGPT1 is studied in angiogenesis, heart development and vascular disease.
Kit Components
| Anti-Mouse Angpt1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse Angpt1 Standard | 2 vials, 10 ng/tube |
| Mouse Angpt1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 156 pg/mL–10,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant mouse Angpt1 (His16–Phe497), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.0–7.7% (3 samples, n = 16 each) |
| Inter-assay CV | 4.9–8.7% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:200. In the supplier’s tests, serum and plasma samples measured about 300 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.047; 156 = 0.106; 312 = 0.164; 625 = 0.262; 1250 = 0.496; 2500 = 0.912; 5000 = 1.609; 10000 = 2.092. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.047 | 0.106 | 0.164 | 0.262 | 0.496 | 0.912 | 1.609 | 2.092 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 351 | 1479 | 5183 | 351 | 1592 | 4701 |
| Standard deviation | 27.02 | 97.61 | 207.32 | 30.53 | 117.8 | 230.34 |
| CV (%) | 7.7% | 6.6% | 4% | 8.7% | 7.4% | 4.9% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Braun Laura J. et al. (2020) Platelets docking to VWF prevent leaks during leukocyte extravasation by stimulating Tie-2. Blood. 10.1182/blood.2019003442
Kenny Schlosser et al. (2018) High circulating angiopoietin-2 levels exacerbate pulmonary inflammation but not vascular leak or mortality in endotoxin-induced lung injury in mice. Thorax. 10.1136/thoraxjnl-2017-210413
Simats Alba et al. (2022) Ceruletide and Alpha-1 Antitrypsin as a Novel Combination Therapy for Ischemic Stroke. Neurotherapeutics. 10.1007/s13311-022-01203-0
Nobuhisa Hirayu et al. (2024) Exploring the Hemostatic Effects of Platelet Lysate-Derived Vesicles: Insights from Mouse Models. International Journal of Molecular Sciences. 10.3390/ijms25021188
Xiang Ou et al. (2019) Angiopoietin-1 aggravates atherosclerosis by inhibiting cholesterol efflux and promoting inflammatory response. Biochimica et Biophysica ACTA-Molecular and Cell Biology of Lipids. 10.1016/j.bbalip.2019.158535
Gul Saba et al. (2021) Whole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malaria. Scientific Reports. 10.1038/s41598-021-91499-3
Burnett Amanda et al. (2017) Angiopoietin-1 enhances neutrophil chemotaxis in vitro and migration in vivo through interaction with CD18 and release of CCL4. Scientific Reports. 10.1038/s41598-017-02216-y
Wenlong Ning et al. (2020) Blocking exosomal miRNA-153-3p derived from bone marrow mesenchymal stem cells ameliorates hypoxia-induced myocardial and microvascular damage by targeting the ANGPT1-mediated VEGF/PI3k/Akt/eNOS pathway. Cellular Signalling. 10.1016/j.cellsig.2020.109812
Gul Saba et al. (2023) Intravenous whole blood transfusion results in faster recovery of vascular integrity and increased survival in experimental cerebral malaria. Memorias Do Instituto Oswaldo Cruz. 10.1590/0074-02760220184
Peng Zheng et al. (2017) Simvastatin ameliorates graft-vs-host disease by regulating angiopoietin-1 and angiopoietin-2 in a murine model. Leukemia Research. 10.1016/j.leukres.2017.01.017
