| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-PE Antibody is an antibody targeting IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel Monoclonal raised in Mouse (R-Phycoerythrin (R-PE)). This antibody is commonly used in IC, IF, IFC, IHC, LCI, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel (also reported as IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel).
- Immunogen/epitope region: Extracellular loop.
- Homology note: Recognizes KCNN4 from human, rat, and mouse samples (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Lot quality control (as provided): Western blot analysis (unlabeled antibody), and direct flow cytometry (labeled antibody)..
- Conjugate/format: R-Phycoerythrin (R-PE) (may affect detection channel and background).
- Antibody type: Monoclonal / IgM.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
KCa3.1 (KCNN4, SK4) is a member of the Ca2+ activated K+ channel family that shares the characteristic of being activated by intracellular Ca2+. The channel has an intermediate conductance, is voltage insensitive and is activated by Ca2+ in the submicromolar range. The channel has a similar topology to that of KV channels, that is, six transmembrane domains and intracellular N- and C-termini.
Research relevance and current trends
- Linking transporter/channel abundance to ionic homeostasis and excitability-related phenotypes.
- Studying compartment-specific localization (surface vs intracellular pools) and trafficking dynamics.
- Combining antibody readouts with functional assays for more complete interpretation.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
- Flow cytometry (direct/indirect): quantify target-positive populations and shifts in expression across subsets.
- Live cell imaging (LCI): support extracellular-epitope detection on non-permeabilized cells when appropriate.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
