| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bone morphogenetic protein 2;BMP-2;Bone morphogenetic protein 2A;BMP-2A;Bmp2;Bmp-2; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: CHO; Immunogen sequence: Q283-R396 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Tissue, Cell Culture Supernatant, Serum |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse bone morphogenetic protein 2 (gene Bmp2) in bone tissue, cell culture supernatants and serum. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Bone morphogenetic protein 2 (BMP2) is a TGF-β superfamily growth factor that binds type I and type II BMP receptors and activates SMAD1/5/8 signaling. It is a strong inducer of bone and cartilage formation and also shapes heart, neural and limb development, which makes it a standard factor in osteogenesis and developmental biology research.
Kit Components
| Anti-Mouse Bmp2 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse Bmp2 Standard | 2 vials, 10 ng/tube |
| Mouse Bmp2 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Bone tissue, cell culture supernatants and serum |
| Assay range | 31.2 pg/mL–2,000 pg/mL |
| Sensitivity | <2 pg/mL |
| Standard | Recombinant mouse Bmp2 (Gln283–Arg396), expressed in CHO |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.1–7.6% (3 samples, n = 16 each) |
| Inter-assay CV | 4.3–7.8% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum samples measured about 1 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.003; 31.2 = 0.071; 62.5 = 0.148; 125 = 0.276; 250 = 0.497; 500 = 0.927; 1000 = 1.44; 2000 = 1.895. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.003 | 0.071 | 0.148 | 0.276 | 0.497 | 0.927 | 1.44 | 1.895 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 62 | 249 | 770 | 61 | 243 | 710 |
| Standard deviation | 2.54 | 10.95 | 58.52 | 2.62 | 12.63 | 55.38 |
| CV (%) | 4.1% | 4.4% | 7.6% | 4.3% | 5.2% | 7.8% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Anxiang Cai et al. (2024) Podocyte Pathogenic Bone Morphogenetic Protein-2 Pathway and Immune Cell Behaviors in Primary Membranous Nephropathy. Advanced Science. 10.1002/advs.202404151
Yizhou Zhu et al. (2021) Regulation of macrophage polarization through surface topography design to facilitate implant-to-bone osteointegration. Science Advances. 10.1126/sciadv.abf6654
Li-Bo Jiang et al. (2021) Injectable sericin based nanocomposite hydrogel for multi-modal imaging-guided immunomodulatory bone regeneration. Chemical Engineering Journal. 10.1016/j.cej.2021.129323
Kathryn Prame Kumar et al. (2024) Stroke Alters the Function of Enteric Neurons to Impair Smooth Muscle Relaxation and Dysregulates Gut Transit. Journal of the American Heart Association. 10.1161/JAHA.123.033279
Ketki Holkar et al. (2025) Microsphere-Mediated Sustained Delivery of Growth Factors Stimulates Osteogenesis in Target Cells in a Three-Dimensional Microenvironment. Journal of Biomedical Materials Research Part a. 10.1002/jbm.a.37947
Yuxian Yan et al. (2016) Mechanical strain promotes osteoblastic differentiation through integrin-β1-mediated β-catenin signaling. International Journal of Molecular Medicine. 10.3892/ijmm.2016.2636
Yong Guo et al. (2015) MicroRNA-218, microRNA-191*, microRNA-3070a and microRNA-33 are responsive to mechanical strain exerted on osteoblastic cells. Molecular Medicine Reports. 10.3892/mmr.2015.3705
Jiawei Jiang et al. (2022) Effects of biomimetic hydroxyapatite coatings on osteoimmunomodulation. Biomaterials Advances. 10.1016/j.msec.2021.112640
