| Field | Specification |
|---|---|
| Mfr No | |
| Assay Type | |
| Detection Wavelength | |
| Product Type | |
| Sample Type(s) | tissue |
| Sensitivity | |
| Species | |
| Storage | |
| Target |
Complement component C5a is a multifunctional proinflammatory mediator produced by complement cascade activation and causes inflammatory responses and cytokine production in septic shock, allergy, trauma, ischemic heart disease, pain, neurodegenerative diseases, and several other autoimmune diseases. C5a is rapidly cleaved to the C5a-DesArg form by the endogenous serum carboxypeptidase enzyme.
Assay Principle
The C5a ELISA Kit can be used for in vitro determination of mouse C5a in different tissue samples including skin, muscle, nervous system and other biological samples. The microplate is coated with murine C5a monoclonal antibody. C5a present in the sample or standard binds to the antibodies absorbed to the microwell. A biotin-conjugated anti-murine C5a antibody is then added and binds to murine C5a captured by the first antibody. After adding streptavidin-horseradish peroxidase (HRP) an antibody-antigen-antibody “sandwich” is produced. Finally, substrate solution reactive with HRP is added to the well, exhibiting a blue color in direct proportion to the amount of C5a in the initial sample. The reaction is terminated by the addition of an acid, and absorbance is measured at 450nm. The concentration of C5a in the samples is determined by comparing the O.D. of the samples to the standard curve.
Mouse C5a ELISA Kit is a Solid-phase sandwich ELISA for the quantitative determination of C5a. The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | C5a |
|---|---|
| Species | Mouse |
| Sample Type | tissue |
| Assay Type | Sandwich |
| Test Principle | Solid-phase sandwich ELISA |
| Sensitivity | < 7.8 pg/mL |
| Detection Wavelength | 450 nm |
| Specificity | This assay recognizes recombinant and natural mouse C5a. |
| Storage | Store the kit at 4°C and standard at -20°C, respectively. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This kit is validated for tissue. Other matrices may require additional in-lab validation.
This assay has a sensitivity of < 7.8 pg/mL. Samples reading outside the range should be diluted or concentrated.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this sandwich ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
