| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-C motif chemokine 1;P500;SIS-epsilon;Small-inducible cytokine A1;T-cell activation protein 3;TCA-3;TCA3;Ccl1;Scya1, Tca3; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: K24-C92 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse C-C motif chemokine 1 (gene Ccl1) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
CCL1 (also called I-309) is a C-C chemokine secreted by activated T cells and other immune cells. It is the ligand for the receptor CCR8 and attracts monocytes and certain T-cell subsets, including regulatory T cells and Th2 cells. It is studied in allergic inflammation and tumor immunology.
Kit Components
| Anti-Mouse Ccl1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse Ccl1 Standard | 2 vials, 10 ng/tube |
| Mouse Ccl1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 7.8 pg/mL–500 pg/mL |
| Sensitivity | <1 pg/mL |
| Standard | Recombinant mouse Ccl1 (Lys24–Cys92), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 5.3–6.8% (3 samples, n = 16 each) |
| Inter-assay CV | 6.4–8.7% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Example standard curve (OD450, pg/mL): 0 = 0.026; 7.8 = 0.108; 15.6 = 0.168; 31.3 = 0.321; 62.5 = 0.627; 125 = 1.043; 250 = 1.676; 500 = 2.23. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.026 | 0.108 | 0.168 | 0.321 | 0.627 | 1.043 | 1.676 | 2.23 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 17 | 81 | 291 | 16 | 78 | 277 |
| Standard deviation | 0.9 | 5.5 | 18.91 | 1.02 | 6.78 | 20.22 |
| CV (%) | 5.3% | 6.8% | 6.5% | 6.4% | 8.7% | 7.3% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Peiqi Yuan et al. (2021) A “sandwich” cell culture platform with NIR-responsive dynamic stiffness to modulate macrophage phenotypes. Biomaterials Science. 10.1039/D0BM02194F
Wen Liu et al. (2020) Blockage of C-C Chemokine Ligand 2 Alleviated the Appendicitis-Induced Injury in Rabbit Model. Journal of Interferon and Cytokine Research. 10.1089/jir.2020.0050
Zivar Yousefipour et al. (2017) Contribution of PPARγ in modulation of acrolein-induced inflammatory signaling in gp91phox knock-out mice. Biochemistry and Cell Biology. 10.1139/bcb-2016-0198
