| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-X-C motif chemokine 5;Cytokine LIX;Small-inducible cytokine B5;GCP-2 (1-78);GCP-2 (9-78);Cxcl5;Scyb5; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: V45-A118 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse C-X-C motif chemokine 5 (gene Cxcl5) in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
CXCL5 (ENA-78) is a CXC chemokine produced by epithelial cells and other cell types during inflammation. It recruits and activates neutrophils through CXCR2 and has angiogenic activity. It is studied in lung and gut inflammation and in tumor biology.
Kit Components
| Anti-Mouse Cxcl5 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse Cxcl5 Standard | 2 vials, 10 ng/tube |
| Mouse Cxcl5 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) |
| Assay range | 15.6 pg/mL–1,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant mouse Cxcl5 (Val45–Ala118), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.7–6.2% (3 samples, n = 16 each) |
| Inter-assay CV | 5.4–6.9% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Example standard curve (OD450, pg/mL): 0 = 0.006; 15.6 = 0.137; 31.2 = 0.214; 62.5 = 0.375; 125 = 0.722; 250 = 1.097; 500 = 1.734; 1000 = 2.209. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.006 | 0.137 | 0.214 | 0.375 | 0.722 | 1.097 | 1.734 | 2.209 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 35 | 166 | 388 | 36 | 150 | 360 |
| Standard deviation | 2.17 | 8.13 | 16.93 | 2.48 | 8.1 | 20.52 |
| CV (%) | 6.2% | 4.9% | 4.7% | 6.9% | 5.4% | 5.7% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Qiangda Chen et al. (2023) Tumor Microenvironment Responsive CD8+ T Cells and Myeloid-Derived Suppressor Cells to Trigger CD73 Inhibitor AB680-Based Synergistic Therapy for Pancreatic Cancer. Advanced Science. 10.1002/advs.202302498
Lisha Zhou et al. (2022) Neddylation pathway promotes myeloid-derived suppressor cell infiltration via NF-κB-mCXCL5 signaling in lung cancer. International Immunopharmacology. 10.1016/j.intimp.2022.109329
Hao-Xiang Yuan et al. (2020) Circulating extracellular vesicles from patients with valvular heart disease induce neutrophil chemotaxis via FOXO3a and the inhibiting role of dexmedetomidine. American Journal of Physiology-Endocrinology and Metabolism. 10.1152/ajpendo.00062.2020
Li He et al. (2020) Protective role of overexpressed MUC5AC against fibrosis in MHV-68-induced combined pulmonary fibrosis and emphysema mouse model. Journal of Medical Virology. 10.1002/jmv.26094
