| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fib2; FG-A; Fibrinopeptide A; Fibrinogen Alpha chain; FGB |
| UniProt # | |
| Reactivity | |
| Applications | |
| Assay type | |
| Sample type(s) | Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific background
FGa(Fibrinogen Alpha) Microsample is a biologically relevant protein marker measured to support mechanistic studies and biomarker discovery (context dependent).
Protein concentrations can change due to secretion, degradation, cell composition shifts, or post-transcriptional regulation, so ELISA readouts often add information beyond gene expression alone.
Quantitative measurements help compare groups and time points using standardized curves and can be interpreted alongside phenotype and pathway-specific readouts.
Why it matters
- Quantify FGa(Fibrinogen Alpha) Microsample to compare biological changes across conditions, doses, or time points.
- Generate concentration data from a standard curve to support biomarker and mechanistic studies.
How the ELISA works
Designed for Mouse samples, this kit uses a The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FGa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FGa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FGa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FGa in the samples is then determined by comparing the OD of the samples to the standard curve.. After binding and washing, signal is converted to concentration using a standard curve.
Sample types: Plasma.
- Detection range: 31.25-2000 ng/mL
- Sensitivity/LoD: 10 ng/mL
- Assay time: 3.5h
It quantifies mouse FGA (fibrinogen alpha chain) in mouse samples.
Validated sample types: plasma.
The kit has a sensitivity of about 10 ng/mL. Dilute samples so readings fall within the standard curve.
It uses a microsample ELISA format.
No. This ELISA kit is for research use only (RUO); it is not intended for diagnostic procedures.
Can?t Find What You?re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
