| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glial cell line-derived neurotrophic factor;mGDNF;Astrocyte-derived trophic factor;ATF;Gdnf; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard:NS0; Immunogen sequence: S78-I211 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse glial cell line-derived neurotrophic factor (gene Gdnf) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Glial cell line-derived neurotrophic factor (GDNF) is a secreted growth factor that strongly supports the survival of dopaminergic and motor neurons. It binds the coreceptor GFRα1 and signals through the RET tyrosine kinase. GDNF is also required for kidney development and spermatogonial stem cell maintenance, and it is studied in Parkinson's disease research.
Kit Components
| Anti-Mouse Gdnf Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse Gdnf Standard | 2 vials, 10 ng/tube |
| Mouse Gdnf Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 31.2 pg/mL–2,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant mouse Gdnf (Ser78–Ile211), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.1–7.5% (3 samples, n = 16 each) |
| Inter-assay CV | 4.2–7.8% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured below the lowest standard.
Example standard curve (OD450, pg/mL): 0 = 0.008; 31.2 = 0.13; 62.5 = 0.207; 125 = 0.333; 250 = 0.57; 500 = 0.958; 1000 = 1.51; 2000 = 2.33. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.008 | 0.13 | 0.207 | 0.333 | 0.57 | 0.958 | 1.51 | 2.33 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 101 | 227 | 789 | 101 | 217 | 855 |
| Standard deviation | 7.57 | 9.3 | 57.53 | 7.67 | 9.11 | 66.69 |
| CV (%) | 7.5% | 4.1% | 7% | 7.6% | 4.2% | 7.8% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Yu-Dong Wei et al. (2021) Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells. Zoological Research. 10.24272/j.issn.2095-8137.2020.186
Yudong Wei et al. (2017) Double sex and mab-3 related transcription factor 1 regulates differentiation and proliferation in dairy goat male germline stem cells. Journal of Cellular Physiology. 10.1002/jcp.26129
Zhang Zhe et al. (2025) Reactive Astrocytes Release GDNF to Promote Brain Recovery and Neuronal Survival Following Ischemic Stroke. Neurochemical Research. 10.1007/s11064-025-04370-6
Wei Liu et al. (2022) Role of GDNF, GFRα1 and GFAP in a Bifidobacterium-Intervention Induced Mouse Model of Intestinal Neuronal Dysplasia. Frontiers in Pediatrics. 10.3389/fped.2021.795678
Oakes Hannah V. et al. (2018) Neurogenesis within the hippocampus after chronic methylphenidate exposure. Journal of Neural Transmission. 10.1007/s00702-018-1949-2
Shijie Song et al. (2016) Granulocyte colony-stimulating factor promotes behavioral recovery in a mouse model of traumatic brain injury. Journal of Neuroscience Research. 10.1002/jnr.23714
Song Shijie et al. (2016) Granulocyte-colony stimulating factor promotes brain repair following traumatic brain injury by recruitment of microglia and increasing neurotrophic factor expression. Restorative Neurology and Neuroscience. 10.3233/RNN-150607
