| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interferon beta; IFN-beta; Ifnb1; Ifb; Ifnb |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: HEK293; Immunogen sequence: I22-N182 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse interferon beta (gene Ifnb1) in cell culture supernatants and serum (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Interferon beta (IFNB1) is a type I interferon made by many cell types, especially fibroblasts and epithelial cells, when they detect viral infection. It binds the IFNAR1/IFNAR2 receptor and activates JAK-STAT signaling to switch on antiviral and immunoregulatory genes, and it is a central cytokine in innate immunity research.
Kit Components
| Anti-Mouse Ifnb1 Pre-coated 96-well Strip Microplate | 12 strips of 8 wells |
|---|---|
| Mouse Ifnb1 Standard | 2 × 4 ng/tube |
| Mouse Ifnb1 Biotinylated Antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Plate Sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, serum (heparin, EDTA) |
| Assay range | 62.5 pg/mL–4,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant mouse Ifnb1 (Ile22–Asn182), expressed in HEK293 |
| Cross-reactivity | No significant cross-reactivity or interference with Ifnb1 analogs in supplier testing; untested analogs cannot be excluded. |
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.008 | 0.1 | 0.17 | 0.297 | 0.537 | 0.97 | 1.806 | 2.443 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 72 | 354 | 1168 | 78 | 459 | 1393 |
| Standard deviation | 5.66 | 22.62 | 67.98 | 3.08 | 36.72 | 91.8 |
| CV(%) | 7.9% | 6.4% | 5.8% | 4% | 8% | 6.6% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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