| Field | Specification |
|---|---|
| Target | |
| Alternative names | Insulin-like growth factor-binding protein 1;IBP-1;IGF-binding protein 1;IGFBP-1;Igfbp1;Igfbp-1; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: A26-N272 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse insulin-like growth factor-binding protein 1 (gene Igfbp1) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
IGFBP-1 is a secreted insulin-like growth factor binding protein produced mainly by the liver and by decidualized endometrium. It binds IGF-1 and IGF-2 and controls their access to receptors, with levels acutely regulated by insulin and nutritional state, and it can also signal through integrin α5β1 to promote cell migration.
Kit Components
| Anti-Mouse Igfbp1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse Igfbp1 Standard | 2 vials, 10 ng/tube |
| Mouse Igfbp1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 31.2 pg/mL–2,000 pg/mL |
| Sensitivity | <3 pg/mL |
| Standard | Recombinant mouse Igfbp1 (Ala26–Asn272), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.4–7.5% (3 samples, n = 16 each) |
| Inter-assay CV | 4.7–8.8% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:10. In the supplier’s tests, serum and plasma samples measured 2737–4530 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.095; 31.2 = 0.137; 62.5 = 0.173; 125 = 0.247; 250 = 0.402; 500 = 0.632; 1000 = 1.22; 2000 = 2.01. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.095 | 0.137 | 0.173 | 0.247 | 0.402 | 0.632 | 1.22 | 2.01 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 88 | 281 | 865 | 90 | 267 | 783 |
| Standard deviation | 6.6 | 14.77 | 38.06 | 7.92 | 19.49 | 36.8 |
| CV (%) | 7.5% | 5.4% | 4.4% | 8.8% | 7.3% | 4.7% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Li Xinzhi et al. (2022) Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH). Nature Communications. 10.1038/s41467-022-32163-w
Lucas M. O’Neill et al. (2022) Hepatic Oleate Regulates Insulin-like Growth Factor-Binding Protein 1 Partially through the mTORC1-FGF21 Axis during High-Carbohydrate Feeding. International Journal of Molecular Sciences. 10.3390/ijms232314671
Matz-Soja Madlen et al. (2014) Hepatic Hedgehog signaling contributes to the regulation of IGF1 and IGFBP1 serum levels. Cell Communication and Signaling. 10.1186/1478-811X-12-11
Shengting Zhang et al. (2013) Distinct post-transcriptional regulation of Igfbp1 gene by hypoxia in lowland mouse and Qinghai-Tibet plateau root vole Microtus oeconomus. Molecular and Cellular Endocrinology. 10.1016/j.mce.2013.05.025
Tania Romacho et al. (2020) DPP4 deletion in adipose tissue improves hepatic insulin sensitivity in diet-induced obesity. American Journal of Physiology-Endocrinology and Metabolism. 10.1152/ajpendo.00323.2019
Koki Tokunaga et al. (2010) Insulin-like growth factor binding protein-1 levels are increased in patients with IgA nephropathy. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2010.07.032
