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Product Overview
Isotype Control
This Mouse IgG1 kappa (IgG1k) isotype control (Clone MOPC-21) is conjugated to PE/TR (Phycoerythrin/Texas Red tandem fluorochrome) and designed as a negative control in Flow Cytometry. The MOPC-21 clone is a well-characterized myeloma-derived IgG1k with no defined antigen specificity — any signal it generates reflects non-specific binding background, not antigen-specific fluorescence.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry | 5 µL/Test (pre-formulated at use concentration) |
Antibody Specifications
| Host Species | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone | MOPC-21 |
| Isotype | IgG1 kappa (IgG1k) |
| Conjugate | PE/TR (Phycoerythrin/Texas Red Tandem Fluorochrome) |
| Purification | Affinity Purified |
| Use Concentration | 5 µL/Test (supplied ready-to-use) |
| Formulation | PBS pH 7.2, 0.09% stabilizer, 1% protein protectant |
| Storage | 2–8°C, protect from light — DO NOT FREEZE |
| Sizes Available | 20 Tests / 100 Tests / 200 Tests (2 × 100) |
| Mfr Catalogue No. | STJA0002722 |
Immunogen & Specificity
Immunogen: No specific antigen — MOPC-21 is a myeloma-derived IgG1k immunoglobulin without defined antigen specificity.
Specificity: None — isotype control. Not designed to bind a specific target antigen.
This antibody is conjugated to PE/TR (Phycoerythrin/Texas Red Tandem) — direct detection without secondary antibody.
Reactivity
Not applicable. Isotype controls assess non-specific antibody binding, not antigen-specific reactivity.
Safety & Handling
Research Use Only (RUO). Not for diagnostic or therapeutic applications. Store at 2–8°C; protect from prolonged light exposure to preserve PE/TR tandem fluorescence. DO NOT FREEZE — tandem dye conjugates lose far-red emission upon freeze-thaw.
Related Products
Browse additional antibodies and detection reagents in the BioHippo catalog.
An isotype control establishes the background fluorescence threshold from non-specific antibody binding (Fc receptor engagement, hydrophobic interactions) — not antigen-specific binding. Clone MOPC-21 carries no antigen specificity, so all signal it produces represents background noise rather than target detection.
Run MOPC-21 IgG1k PE/TR at 5 µL/Test — the same concentration as your specific PE/TR-conjugated mouse IgG1k antibody of interest — on the same cell population with the same staining protocol. Set your positive gate just above the upper boundary of the MOPC-21 fluorescence distribution.
This antibody is conjugated to a PE/TR tandem fluorochrome. Freezing disrupts the energy transfer between PE (donor) and Texas Red (acceptor) chromophores, resulting in loss of far-red (Texas Red channel) emission and appearance of spectral artifacts in the PE channel. Store at 2–8°C only.
This product is available in: 20 Tests (STJA0002722-20), 100 Tests (STJA0002722-100), and 200 Tests/2 × 100 Tests (STJA0002722-200). All sizes are pre-formulated at 5 µL/Test — no further dilution is needed.
PE/TR (Phycoerythrin/Texas Red tandem) is excited by the 488 nm blue laser. Emission peaks in the Texas Red channel at ~613–615 nm. Use a 610/20 or 613/20 bandpass filter for optimal detection on a standard flow cytometer equipped with a 488 nm laser.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
