| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-23 subunit alpha;IL-23 subunit alpha;IL-23-A;Interleukin-23 subunit p19;IL-23p19;Il23a; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: (M23-S335) + (L20-A196) |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse interleukin-23 subunit alpha (gene Il23a) in cell culture supernatants, serum and plasma (heparin). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
IL-23 p19 (IL23A) is the subunit that combines with p40 (IL12B) to form the cytokine IL-23, released by dendritic cells and macrophages. IL-23 signals through IL-12Rβ1 and IL-23R to activate JAK2, TYK2 and STAT3, supporting the expansion and survival of IL-17–producing Th17 cells. It is a central target in studies of chronic inflammation and autoimmunity.
Kit Components
| Anti-Mouse Il23a Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse Il23a Standard | 2 vials, 10 ng/tube |
| Mouse Il23a Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (heparin) |
| Assay range | 31.2 pg/mL–2,000 pg/mL |
| Sensitivity | <2 pg/mL |
| Standard | Recombinant mouse Il23a ((M23-S335)), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.4–5.0% (3 samples, n = 16 each) |
| Inter-assay CV | 5.1–6.9% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured below the lowest standard.
Example standard curve (OD450, pg/mL): 0 = 0.043; 31.2 = 0.115; 62.5 = 0.175; 125 = 0.268; 250 = 0.446; 500 = 0.823; 1000 = 1.418; 2000 = 2.273. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.043 | 0.115 | 0.175 | 0.268 | 0.446 | 0.823 | 1.418 | 2.273 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 93 | 278 | 780 | 85 | 279 | 762 |
| Standard deviation | 4.09 | 13.9 | 34.32 | 4.4%7 | 19.25 | 38.86 |
| CV (%) | 4.4% | 5% | 4.4% | 6.2% | 6.9% | 5.1% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Ma Xiaolu et al. (2024) Foamed microemulsion nanodroplets loaded with chlorin e6 for epidermal-targeted treatment against psoriasis. Nano Research. 10.1007/s12274-024-6916-7
Lulu Chu et al. (2025) Protective effects of carboxymethyl chitosan-dialdehyde glucan/polydopamine carrier-delivered Bacillus subtilis on the intestinal tract of mice infected with Salmonella. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2025.143316
Yuchao Chen et al. (2024) Percutaneous Delivery of Hederacoside C-Loaded Nanoliposome Gel Alleviates Psoriasiform Skin Inflammation through the CCL17/Treg Axis. ACS Applied Materials & Interfaces. 10.1021/acsami.4c06720
Wang Jing et al. (2021) Bazedoxifene Regulates Th17 Immune Response to Ameliorate Experimental Autoimmune myocarditis via Inhibition of STAT3 Activation. Frontiers in Pharmacology. 10.3389/fphar.2020.613160
Changhui Wen et al. (2026) Integrating network pharmacology and experimental validation to elucidate the mechanism of Xiao-bi decoction in psoriasis treatment: Inhibition of JAK2/STAT3 signaling and rebalancing Th17/Treg responses. Journal of Ethnopharmacology. 10.1016/j.jep.2026.121477
Bolin Li et al. (2021) Luteolin alleviates inflammation and modulates gut microbiota in ulcerative colitis rats. Life Sciences. 10.1016/j.lfs.2020.119008
Mengxi Ying et al. (2020) Ganoderma atrum polysaccharide ameliorates intestinal mucosal dysfunction associated with autophagy in immunosuppressed mice. Food and Chemical Toxicology. 10.1016/j.fct.2020.111244
Liu Ke et al. (2024) Indomethacin Combined with Ciprofloxacin Improves the Prognosis of Mice under Severe Traumatic Infection via the PI3K/Akt Pathway in Macrophages. Inflammation. 10.1007/s10753-024-02008-3
Hongqing Zhu et al. (2018) Structure elucidation and bioactivities of a new polysaccharide from Xiaojin Boletus speciosus Frost. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2018.12.216
Wen-Cheng Chen et al. (2023) IL-23/IL-17 immune axis mediates the imiquimod-induced psoriatic inflammation by activating ACT1/TRAF6/TAK1/NF-κB pathway in macrophages and keratinocytes. Kaohsiung Journal of Medical Sciences. 10.1002/kjm2.12683
