| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin 6 ;Interleukin 6, isoform CRA_b ;IL6 ;mCG_11634 ; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: F25-T211 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse interleukin-6 (gene Il6) in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Interleukin-6 (IL-6) is a multifunctional cytokine released by macrophages, dendritic cells and many other cells during infection and tissue injury. It binds IL-6R and then the signaling subunit gp130, activating JAK–STAT3 through classic or trans-signaling. IL-6 drives the acute phase response, B-cell differentiation and Th17 development, and it is one of the most widely measured inflammatory markers.
Kit Components
| Anti-Mouse IL6 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse IL6 Standard | 2 vials, 10 ng/tube |
| Mouse IL6 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) |
| Assay range | 1.56–100 pg/mL |
| Sensitivity | <0.17 pg/mL |
| Standard | Recombinant mouse Il6 (Phe25–Thr211), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Format | 96 wells, removable strips |
Example standard curve (OD450, pg/mL): 0 = 0.03; 1.56 = 0.117; 3.12 = 0.172; 6.25 = 0.315; 12.5 = 0.506; 25 = 0.939; 50 = 1.775; 100 = 2.481. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.03 | 0.117 | 0.172 | 0.315 | 0.506 | 0.939 | 1.775 | 2.481 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 3 | 9 | 29 | 2 | 10 | 27 |
| Standard deviation | 0.23 | 0.58 | 1.27 | 0.05 | 0.65 | 1.59 |
| CV (%) | 7.7% | 6.5% | 4.4% | 2.6% | 6.5% | 5.9% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Yiming Li et al. (2025) Skin-Derived Precursors-Derived Exosomes Alleviated Acne Inflammation via TLR2/MyD88/NF-κB Signaling Pathway: In Vitro and In Vivo Studies. FASEB Journal. 10.1096/fj.202501944R
