| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | BPY2IP1; C19orf5; FLJ10669; MAP8; MGC133087; VCY2IP-1; VCY2IP1; BPY2 interacting protein 1|VCY2 interacting protein 1|microtubule-associated protein 8 |
| Product Type | |
| Sensitivity | |
| UniProt # |
Scientific Background
Using the SEC1 homology domain of LRPPRC as probe in a yeast 2-hybrid screen, Liu and McKeehan (2002) cloned VCY2IP1 (BPY2IP1), which they called C19ORF5, from a liver cDNA library.The deduced 672-amino acid protein is proline-rich (12.8%) and contains an arginine-rich potential RNA-binding motif. VCY2IP1 shares significant homology with several microtubule-associated proteins. Northern blot analysis revealed a major transcript of about 3 kb in all 12 tissues tested. Additional less-abundant transcripts were detected in some tissues.The International Radiation Hybrid Mapping Consortium mapped.
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate MAP1S in samples. An antibody specific for MAP1S has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAP1S present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP1S is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP1S bound in the initial step. The color development is stopped and the intensity of the color is measured.
Performance Specifications
| Sensitivity | Request Information |
|---|---|
| Detection Range | Request Information |
| Total Assay Time | 3.5–5 hours |
| Compatible Sample Types | serum, plasma, cell culture supernatant, tissue homogenate |
| Species Reactivity | Mouse |
| Detection Method | Colorimetric (TMB/HRP) |
| Storage | 4°C (short-term); -20°C (long-term) |
✓ Research-Grade Validation
Safety & Regulatory
Handle reagents in accordance with institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for complete hazard and handling information. Contains components that may require special disposal procedures per local regulations.
This kit is validated for use with serum, plasma, cell culture supernatant, tissue homogenate. For unlisted matrices (e.g., tissue lysate, urine), perform a spike-and-recovery experiment to confirm assay performance before generating reportable data. Sample dilution in the kit's provided diluent is recommended to minimize matrix interference.
The minimum detectable concentration (sensitivity) of this kit is Request Information. Values below this threshold should be reported as below the limit of detection (<LOD) and should not be extrapolated from the standard curve.
The total assay time from sample addition to absorbance reading is approximately 3.5–5 hours, including all incubation, wash, and substrate steps. Hands-on time is typically 1–2 hours; most steps involve passive plate incubation. Plan the assay as a single uninterrupted session for best results.
Standard components of this Sandwich ELISA Kit typically include: pre-coated microplate (96-well strip format), lyophilized or liquid recombinant MAP1S standard, detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, wash buffer concentrate, and sample/standard diluent. Refer to the kit insert or datasheet for the exact component list and storage requirements.
This kit uses colorimetric (TMB/HRP) detection and requires a standard microplate absorbance reader capable of measuring at 450 nm. A reference wavelength of 570 nm or 630 nm is recommended to reduce background. No specialized fluorescence or luminescence reader is needed. Ensure the instrument is calibrated and the plate is clean and free of condensation before reading.
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