| Field | Specification |
|---|---|
| Target | |
| Alternative names | Macrophage metalloelastase;MME;3.4.24.65;Matrix metalloproteinase-12;MMP-12;Mmp12;Mme, Mmel; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: A18-C462 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse macrophage metalloelastase (gene Mmp12) in cell culture supernatants, serum and plasma (heparin). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Macrophage metalloelastase (MMP-12) is a matrix metalloproteinase produced mainly by macrophages that breaks down elastin and other matrix proteins. It is widely studied in lung tissue injury and remodeling, especially in models of emphysema and chronic inflammation.
Kit Components
| Anti-Mouse Mmp12 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse Mmp12 Standard | 2 vials, 10 ng/tube |
| Mouse Mmp12 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (heparin) |
| Assay range | 62.5 pg/mL–4,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant mouse Mmp12 (Ala18–Cys462), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 7.0–7.4% (3 samples, n = 16 each) |
| Inter-assay CV | 7.1–8.4% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured about 100 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.054; 62.5 = 0.19; 125 = 0.299; 250 = 0.505; 500 = 0.81; 1000 = 1.285; 2000 = 1.801; 4000 = 2.101. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.054 | 0.19 | 0.299 | 0.505 | 0.81 | 1.285 | 1.801 | 2.101 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 127 | 628 | 1430 | 134 | 684 | 1370 |
| Standard deviation | 9.27 | 46.47 | 100.1 | 11.12 | 57.45 | 97.27 |
| CV (%) | 7.3% | 7.4% | 7% | 8.3% | 8.4% | 7.1% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Qian Huang et al. (2024) DACH1 Attenuates Airway Inflammation in COPD by Activating NRF2 Signaling. American Journal of Respiratory Cell and Molecular Biology. 10.1165/rcmb.2023-0337OC
Takimoto-Sato Michiko et al. (2023) Apoptosis inhibitor of macrophage (AIM)/CD5L is involved in the pathogenesis of COPD. Respiratory Research. 10.1186/s12931-023-02508-0
Na Zang et al. (2016) Pulmonary C Fibers Modulate MMP-12 Production via PAR2 and Are Involved in the Long-Term Airway Inflammation and Airway Hyperresponsiveness Induced by Respiratory Syncytial Virus Infection. Journal of Virology. 10.1128/JVI.02534-15
Shoji Suzuki et al. (2020) ADAM17 protects against elastase-induced emphysema by suppressing CD62L+ leukocyte infiltration in mice. American Journal of Physiology-Lung Cellular and Molecular Physiology. 10.1152/ajplung.00214.2019
