| Field | Specification |
|---|---|
| Target | |
| Alternative names | ARIA; GGF; GGF2; HGL; HRG; HRG1; HRGA; NDF; SMDF; glial growth factor|heregulin; alpha (45kD; ERBB2 p185-activator)|neu differentiation factor|sensory and motor neuron derived factor |
| Species | |
| Applications | |
| Assay type | |
| Sensitivity | |
| Detection range | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Neuregulin 1 or NRG1 is one of the four proteins of the neuregulin family which act on EGFR family of receptors. Neuregulin 1 is produced in numerous isoforms by alternative splicing, and this allows it to perform a wide variety of functions. Neuregulin 1-ErbB4 interactions are thought to play a role in the pathological mechanism of schizophrenia. The protein also has a putative ability to protect the brain from damage induced by stroke. Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing.
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate NRG1 in samples. An antibody specific for NRG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNRG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NRG1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NRG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | 15.6-1000 pg/mL |
| Sensitivity | 6.2 pg/mL |
| Species Reactivity | Mouse (Mus musculus) |
| Stability | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C). |
| Manufacturer No. | AE34857MO |
| Alternative Names | ARIA; GGF; GGF2; HGL; HRG; HRG1; HRGA; NDF; SMDF; glial growth factor|heregulin; alpha (45kD; ERBB2 p185-activator)|neu differentiation factor|sensory and motor neuron derived factor |
Assay Workflow
The kit includes the components required for standard-curve quantification (see the kit manual for the component table and validated sample types). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.
Safety & Handling
For research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional Mouse ELISA kits from Wuhan Abebio Science Co Ltd, or contact us for related assays for NRG1 research.
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