| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor necrosis factor receptor superfamily member 11B;Osteoclastogenesis inhibitory factor;Osteoprotegerin;Tnfrsf11b;Ocif, Opg; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: E22-L401 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse tumor necrosis factor receptor superfamily member 11B (gene Tnfrsf11b) in cell culture supernatants, serum and plasma (EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Osteoprotegerin (OPG, TNFRSF11B) is a secreted decoy receptor that binds RANKL and blocks its interaction with RANK. This prevents osteoclast formation and activation, so the local RANKL/OPG ratio is a key determinant of bone mass. OPG can also bind TRAIL and is studied in bone metabolism, vascular calcification and cancer.
Kit Components
| Anti-Mouse Tnfrsf11b Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse Tnfrsf11b Standard | 2 vials, 10 ng/tube |
| Mouse Tnfrsf11b Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (EDTA) |
| Assay range | 93.7 pg/mL–6,000 pg/mL |
| Sensitivity | <5 pg/mL |
| Standard | Recombinant mouse Tnfrsf11b (Glu22–Leu401), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.4–6.8% (3 samples, n = 16 each) |
| Inter-assay CV | 4.6–7.3% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured about 400 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.014; 93.8 = 0.071; 187.5 = 0.138; 375 = 0.275; 750 = 0.536; 1500 = 0.92; 3000 = 1.532; 6000 = 2.158. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.014 | 0.071 | 0.138 | 0.275 | 0.536 | 0.92 | 1.532 | 2.158 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 133 | 800 | 3535 | 139 | 767 | 3643 |
| Standard deviation | 5.85 | 54.4 | 187.35 | 7.39 | 55.39 | 225.86 |
| CV (%) | 4.4% | 6.8% | 5.3% | 4.6% | 7.3% | 6.2% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Vishwa Deepak et al. (2022) IFT80 negatively regulates osteoclast differentiation via association with Cbl-b to disrupt TRAF6 stabilization and activation. Proceedings of the National Academy of Sciences of the United States of America. 10.1073/pnas.2201490119
Yang Li et al. (2022) IFT20 governs mesenchymal stem cell fate through positively regulating TGF-β-Smad2/3-Glut1 signaling mediated glucose metabolism. Redox Biology. 10.1016/j.redox.2022.102373
Hongyu Li et al. (2021) Introduction of a Cys360Tyr Mutation in ANO5 Creates a Mouse Model for Gnathodiaphyseal Dysplasia. Journal of Bone and Mineral Research. 10.1002/jbmr.4481
Liu Hui et al. (2021) Inhibiting Monoacylglycerol Lipase Suppresses RANKL-Induced Osteoclastogenesis and Alleviates Ovariectomy-Induced Bone Loss. Frontiers in Cell and Developmental Biology. 10.3389/fcell.2021.640867
Liu Hui et al. (2020) NR1D1 modulates synovial inflammation and bone destruction in rheumatoid arthritis. Cell Death & Disease. 10.1038/s41419-020-2314-6
Fangji Yang et al. (2022) NTCP Deficiency Affects the Levels of Circulating Bile Acids and Induces Osteoporosis. Frontiers in Endocrinology. 10.3389/fendo.2022.898750
Chao Song et al. (2018) REV-ERB agonism suppresses osteoclastogenesis and prevents ovariectomy-induced bone loss partially viaFABP4 upregulation. FASEB Journal. 10.1096/fj.201600825RRR
Zhang Yong et al. (2015) Amlexanox Suppresses Osteoclastogenesis and Prevents Ovariectomy-Induced Bone Loss. Scientific Reports. 10.1038/srep13575
Hanfeng Guan et al. (2014) Epoxyeicosanoids suppress osteoclastogenesis and prevent ovariectomy-induced bone loss. FASEB Journal. 10.1096/fj.14-262055
Wang Zhihang et al. (2025) Up-regulation of Ifi27l2a expression in bone marrow monocytes contributes to glucocorticoid-induced bone loss. Communications Biology. 10.1038/s42003-025-08985-x
