| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sparc ELISA Kit; SPARC ELISA Kit; Basement-membrane protein 40 ELISA Kit; BM-40 ELISA Kit; Osteonectin ELISA Kit; ON ELISA Kit; Secreted protein acidic and rich in cysteine ELISA Kit |
| UniProt # | |
| Species | |
| Reactivity | |
| Applications | |
| Assay type | |
| Sample type(s) | serum, plasma, cell culture supernates, tissue homogenates |
| Sensitivity | |
| Detection range | |
| Detection wavelength | |
| Assay time | |
| Catalog no. (Mfr.) | |
| Main SKU |
Background
osteonectin (SPARC) is a biological molecule commonly studied in cancer research. It is commonly used as a molecular readout in mechanistic and biomarker-focused studies.
UniProt: P07214
Biological context
Researchers often monitor osteonectin in serum, plasma, cell culture supernates, and tissue homogenates to better understand themes such as tumor microenvironment biology, cell proliferation and apoptosis, and metastasis and invasion pathways. In many model systems, measured levels can shift with physiology, experimental perturbation, or disease-associated changes, making careful biological interpretation important.
Interpreting changes in measured levels
Depending on sample matrix and study design, increases or decreases in osteonectin may reflect differences in expression, secretion, turnover, or compartmentalization rather than a single mechanism. Interpretation is typically strengthened by evaluating related molecules (for example, cell-cycle regulators, invasion/ECM markers, and immune-oncology mediators) and by keeping pre-analytical variables consistent across groups.
Nomenclature
In publications and databases, osteonectin may also appear under names such as Sparc and Basement-membrane protein 40. When comparing studies, confirm that the reported analyte refers to the same molecule and species context.
Why ELISA data are widely used
ELISA is a common approach for quantitative measurement of proteins and biomarkers in complex samples, enabling comparisons across experimental groups and time points. When integrating results with other readouts, consider species biology, sample type, and the broader pathway context that osteonectin participates in.
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