| Field | Specification |
|---|---|
| Target | |
| Alternative names | P-selectin;CD62 antigen-like family member P;Granule membrane protein 140;GMP-140;Leukocyte-endothelial cell adhesion molecule 3;LECAM3;Platelet activation dependent granule-external membrane protein;PADGEM;CD62P;Selp;Grmp; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: W42-A709 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse P-selectin (gene Selp) in cell culture supernatants, serum and plasma (citrate). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
P-selectin (SELP, CD62P) is an adhesion molecule stored in platelet α-granules and endothelial Weibel-Palade bodies and moved to the cell surface upon activation. It binds PSGL-1 on leukocytes to start their rolling during inflammation and links platelets with leukocytes, and it is widely used as a platelet activation marker.
Kit Components
| Anti-Mouse Selp Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse Selp Standard | 2 vials, 20 ng/tube |
| Mouse Selp Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (citrate) |
| Assay range | 312 pg/mL–20,000 pg/mL |
| Sensitivity | <5 pg/mL |
| Standard | Recombinant mouse Selp (Trp42–Ala709), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.8–5.9% (3 samples, n = 16 each) |
| Inter-assay CV | 5.0–6.7% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Example standard curve (OD450, ng/mL): 0 = 0.025; 0.312 = 0.195; 0.62 = 0.311; 1.25 = 0.514; 2.5 = 0.801; 5 = 1.312; 10 = 1.763; 20 = 2.029. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (ng/ml) | 0 | 0.312 | 0.62 | 1.25 | 2.5 | 5 | 10 | 20 |
| O.D. | 0.025 | 0.195 | 0.311 | 0.514 | 0.801 | 1.312 | 1.763 | 2.029 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 596 | 2016 | 7521 | 640 | 1834 | 7467 |
| Standard deviation | 28.6 | 106.84 | 443.73 | 32 | 105% | 500.28 |
| CV (%) | 4.8% | 5.3% | 5.9% | 5% | 5.9% | 6.7% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Liangzhu Li et al. (2019) Recruitment of HIV-1 target cells at topical mucosal sites: a sensitive and early marker for determining the safety of microbicide candidates. Emerging Microbes & Infections. 10.1038/emi.2013.42
Xin Tun et al. (2014) Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis. Free Radical Biology and Medicine. 10.1016/j.freeradbiomed.2014.06.020
Jing Guo et al. (2024) Icariin ameliorates LPS-induced acute lung injury in mice via complement C5a-C5aR1 and TLR4 signaling pathways. International Immunopharmacology. 10.1016/j.intimp.2024.111802
Li Jiao et al. (2024) Baicalin relieves complement alternative pathway activation-induced lung inflammation through inhibition of NF-κB pathway. BMC Complementary Medicine and Therapies. 10.1186/s12906-024-04622-y
Keiji Yasukawa et al. (2012) The detrimental effect of nitric oxide on tissue is associated with inflammatory events in the vascular endothelium and neutrophils in mice with dextran sodium sulfate-induced colitis. Free Radical Research. 10.3109/10715762.2012.732698
Guo Jing et al. (2020) Pretreatment with atorvastatin ameliorates cobra venom factor-induced acute lung inflammation in mice. BMC Pulmonary Medicine. 10.1186/s12890-020-01307-3
Zhao Yilin et al. (2014) Simvastatin Inhibits Inflammation in Ischemia-Reperfusion Injury. Inflammation. 10.1007/s10753-014-9918-x
Yong‑Qiang Bai et al. (2016) Effects of the eukaryotic initiation factor 6 gene on expression levels of inflammatory mediators in M2 macrophages during scar repair Retraction in /10.3892/mmr.2017.7190. Molecular Medicine Reports. 10.3892/mmr.2016.5294
