| Field | Specification |
|---|---|
| Target | |
| Alternative names | Proprotein convertase subtilisin/kexin type 9;3.4.21.-;Neural apoptosis-regulated convertase 1;NARC-1;Proprotein convertase 9;PC9;Subtilisin/kexin-like protease PC9;Pcsk9;Narc1; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: S156-Q694 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse proprotein convertase subtilisin/kexin type 9 (gene Pcsk9) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
PCSK9 is a secreted proprotein convertase made mainly in the liver that binds the LDL receptor and sends it for lysosomal degradation. By lowering LDL receptor levels it raises plasma LDL cholesterol, which made PCSK9 a major target of cholesterol-lowering antibodies and siRNA drugs.
Kit Components
| Anti-Mouse Pcsk9 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse Pcsk9 Standard | 2 vials, 10 ng/tube |
| Mouse Pcsk9 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 156 pg/mL–10,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant mouse Pcsk9 (Ser156–Gln694), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.2–7.8% (3 samples, n = 16 each) |
| Inter-assay CV | 5.3–9.6% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:100. In the supplier’s tests, serum and plasma samples measured 100–150 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.006; 156 = 0.092; 312 = 0.158; 625 = 0.273; 1250 = 0.508; 2500 = 0.907; 5000 = 1.647; 10000 = 2.24. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.006 | 0.092 | 0.158 | 0.273 | 0.508 | 0.907 | 1.647 | 2.24 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 95 | 662 | 1355 | 93 | 670 | 1497 |
| Standard deviation | 3.99 | 48.33 | 105.69 | 4.93 | 48.91 | 143.71 |
| CV (%) | 4.2% | 7.3% | 7.8% | 5.3% | 7.3% | 9.6% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Anthony Conway et al. (2019) Non-viral Delivery of Zinc Finger Nuclease mRNA Enables Highly Efficient In Vivo Genome Editing of Multiple Therapeutic Gene Targets. Molecular Therapy. 10.1016/j.ymthe.2019.03.003
Mei Feng et al. (2025) Liver-targeted siRNA delivery via artificial red blood cells to lower LDL-C. International Journal of Pharmaceutics. 10.1016/j.ijpharm.2025.126156
Rohrbach Susanne et al. (2021) Impact of PCSK9 on CTRP9-Induced Metabolic Effects in Adult Rat Cardiomyocytes. Frontiers in Physiology. 10.3389/fphys.2021.593862
Feifei Wang et al. (2022) PCSK9 Modulates Macrophage Polarization-Mediated Ventricular Remodeling after Myocardial Infarction. Journal of Immunology Research. 10.1155/2022/7685796
