| Field | Specification |
|---|---|
| Target | |
| Alternative names | Retinol-binding protein 4 ;Rbp4 ; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: E19-L201 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma, Urine |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse retinol-binding protein 4 (gene Rbp4) in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Retinol-binding protein 4 (RBP4) is the main plasma carrier of vitamin A. Produced mostly by the liver, it transports retinol in blood, often in complex with transthyretin, and delivers it to tissues through the receptor STRA6. RBP4 is also released by fat tissue and is measured in studies of vitamin A status, insulin resistance and metabolic disease.
Kit Components
| Anti-Mouse Rbp4 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse Rbp4 Standard | 2 vials, 20 ng/tube |
| Mouse Rbp4 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, serum, plasma (heparin, EDTA) and urine |
| Assay range | 312 pg/mL–20,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant mouse Rbp4 (Glu19–Leu201), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.5–7.7% (3 samples, n = 16 each) |
| Inter-assay CV | 6.2–8.3% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:500. In the supplier’s tests, serum and plasma samples measured 1–2 µg/mL.
Example standard curve (OD450, ng/mL): 0 = 0.022; 0.312 = 0.087; 0.62 = 0.133; 1.25 = 0.228; 2.5 = 0.411; 5 = 0.747; 10 = 1.44; 20 = 2.227. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (ng/ml) | 0 | 0.312 | 0.62 | 1.25 | 2.5 | 5 | 10 | 20 |
| O.D. | 0.022 | 0.087 | 0.133 | 0.228 | 0.411 | 0.747 | 1.44 | 2.227 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 418 | 2343 | 8435 | 401 | 2576 | 7635 |
| Standard deviation | 23.4 | 105.43 | 649.49 | 28.07 | 159.71 | 633.7 |
| CV (%) | 4.5% | 4.5% | 7.7% | 7% | 6.2% | 8.3% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Sheng Zhang et al. (2021) Proteomics analysis in the kidney of mice following oral feeding Realgar. Journal of Ethnopharmacology. 10.1016/j.jep.2021.114118
Lin Tian et al. (2008) Diabetes-induced upregulation of urotensin II and its receptor plays an important role in TGF-β1-mediated renal fibrosis and dysfunction. American Journal of Physiology-Endocrinology and Metabolism. 10.1152/ajpendo.90672.2008
Zhiwei Xu et al. (2022) Elevated retinal retinol-binding protein 4 levels in diabetic mice can induce retinal neurodegeneration through microglia. Microscopy Research and Technique. 10.1002/jemt.24258
