| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein S100-A9;Calgranulin-B;Leukocyte L1 complex heavy chain;Migration inhibitory factor-related protein 14;MRP-14;p14;S100 calcium-binding protein A9;S100a9;Cagb, Mrp14; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: A2-K113 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse protein S100-A9 (gene S100a9) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
S100A9 (also called calgranulin B or MRP14) is a calcium- and zinc-binding protein that forms calprotectin with S100A8 in neutrophils and monocytes. It supports phagocyte migration, NADPH oxidase activity and neutrophil degranulation, and once released it signals as an alarmin, making it a widely studied inflammatory marker.
Kit Components
| Anti-Mouse S100a9 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse S100a9 Standard | 2 vials, 2 ng/tube |
| Mouse S100a9 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 31.2 pg/mL–2,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant mouse S100a9 (Ala2–Lys113), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.3–5.5% (3 samples, n = 16 each) |
| Inter-assay CV | 5.6–7.0% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured below the lowest standard.
Example standard curve (OD450, pg/mL): 0 = 0.05; 31.2 = 0.127; 62.5 = 0.193; 125 = 0.316; 250 = 0.528; 500 = 0.901; 1000 = 1.579; 2000 = 2.555. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.05 | 0.127 | 0.193 | 0.316 | 0.528 | 0.901 | 1.579 | 2.555 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 104 | 235 | 1022 | 110 | 231 | 1104 |
| Standard deviation | 4.47 | 12.92 | 50.07 | 6.16 | 16.17 | 64.03 |
| CV (%) | 4.3% | 5.5% | 4.9% | 5.6% | 7% | 5.8% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Wen Guo et al. (2025) NO-releasing double-crosslinked responsive hydrogels accelerate the treatment and repair of ischemic stroke. Acta Pharmaceutica Sinica B. 10.1016/j.apsb.2025.01.005
Huo Shengqi et al. (2025) Macrophage-derived S100A9 promotes diabetic cardiomyopathy by disturbing mitochondrial quality control via STAT3 activation. International Journal of Biological Sciences. 10.7150/ijbs.111128
Ning Yang et al. (2026) Macrophage-derived S100A9 drives liver fibrosis in Echinococcus multilocularis infection by up-regulating secreted protein acidic and rich in cysteine (SPARC) in hepatic stellate cells. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2026.150624
Sharan R et al. (2016) Interleukin-22 protects against non-typeable Haemophilus influenzae infection: alteration during chronic obstructive pulmonary disease. Mucosal Immunology. 10.1038/mi.2016.40
Zhu Wei et al. (2023) Acetate attenuates hyperoxaluria-induced kidney injury by inhibiting macrophage infiltration via the miR-493-3p/MIF axis. Communications Biology. 10.1038/s42003-023-04649-w
Moran Wang et al. (2025) S100A9 inhibition ameliorates HFpEF by modulating mitochondrial fission and oxidative stress. International Immunopharmacology. 10.1016/j.intimp.2025.115280
Han Li et al. (2019) S100-A9 protein in exosomes derived from follicular fluid promotes inflammation via activation of NF-κB pathway in polycystic ovary syndrome. Journal of Cellular and Molecular Medicine. 10.1111/jcmm.14642
Jinfeng Cui et al. (2025) Proteomics suggests the role of Cxcl12 secreted by hucMSCs in the treatment of lipopolysaccharide-acute lung injury. Microvascular Research. 10.1016/j.mvr.2025.104815
