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Product Overview
A telomere is a DNA sequence at the end of a eukaryotic chromosome composed of multiple repeating nucleotide elements (TTAGGG) in tandem. In addition to providing a buffer for non-transcribed DNA, it also protects chromosome ends from fusion and degradation, preserving chromosome stability and genetic integrity. Telomere length is determined initially by genetic and environmental factors and decreases over time. Studies have shown that telomere length is closely related to DNA repair, aging, apoptosis, and tumorigenesis. Therefore, accurate and repeatable measurement of telomere length is particularly important for researchers. This product mainly uses relative quantitative qPCR to directly compare the average telomere length of the sample, that is, the copy number ratio (T/S) of the telomere repeat sequence (Tel) and the genome single copy gene (SCR) copy number (T/S) is used as the telomere relative length. Single-copy gene primers (SCR) specifically recognize and amplify the 100 bp long region on mouse chromosome 10. The primer set in the kit has passed the test to ensure: (1) efficient and reliable quantification; (2) no non-specific amplification. Each set of primers has been verified by amplification curve efficiency (E>98%, R²>0.99), melting curve analysis and gel electrophoresis verification.
The assay is run in a 20 µL reaction with 0.5–5 ng of genomic DNA. Cycling is 95°C for 3 min, then 35–40 cycles of 95°C for 5 sec, 58°C for 30 sec and 72°C for 30 sec, followed by melting curve analysis. Quantification uses the comparative ∆∆Cq method.
Specifications
| Method | Relative quantitative qPCR with melting curve analysis |
|---|---|
| Target | Telomere repeat sequence (Tel) against a genomic single-copy reference gene (SCR) |
| Species | Mouse |
| Reference gene region | 100 bp region on mouse chromosome 10 |
| Sample input | 0.5–5 ng genomic DNA per 20 µL reaction |
| Primer performance | E>98%, R²>0.99 |
| Result principle | Comparative ∆∆Cq |
| ROX | 50× ROX supplied as a separate vial |
| Reactions per kit | 100 rxn or 500 rxn |
Kit Components
| Component | 100 rxn | 500 rxn |
|---|---|---|
| 2× Mix | 1 mL | 1.25 mL × 4 |
| Telomere Primer Mix (10 µM) | 50 µL | 100 µL |
| Single Copy Reference Primer Mix (10 µM) | 50 µL | 100 µL |
| 50× ROX | 250 µL | 1.25 mL |
| RNase-free ddH₂O | 1 mL | 1.25 mL × 4 |
Applications
- Relative telomere length measurement in mouse samples
- Comparison of average telomere length between samples
Storage & Handling
Stored at -20°C and protected from light, with a shelf life of one year. Shipping: Ice pack or dry ice.
Usage Notes
- A minimum of three biological replicates is required.
- Avoid strong light exposure during storage and reaction preparation.
- Confirm the amplification and melting curves after the reaction.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
