| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Puromycin, Blasticidin, N/A |
| Shipping | |
| Species |
Background
Tfrc encodes the transferrin receptor 1 (TfR1, CD71), a transmembrane glycoprotein that mediates cellular iron uptake by binding iron-loaded transferrin and internalizing it through receptor-mediated endocytosis. As the principal route of iron acquisition, TfR1 is essential for processes that depend on iron, including DNA synthesis, mitochondrial respiration, and erythropoiesis. Its expression is tightly coupled to cellular iron demand and proliferative state, and is markedly elevated in many rapidly dividing and tumor cells. Because of this, TfR1 is widely studied as a marker of proliferation and iron metabolism, as a determinant of ferroptosis sensitivity, and as a candidate target for cancer therapy and drug delivery.
Product Description & Applications
The Mouse Tfrc shRNA Lentivirus delivers a validated short hairpin RNA that achieves at least 70% knockdown of mouse transferrin receptor (Tfrc/CD71). The shRNA is expressed from a U6 promoter in a third-generation, self-inactivating lentiviral backbone, with a co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and antibiotic selection marker enabling stable cell line generation. Knockdown efficiency is confirmed using a rapid fluorescence-based assay rather than qPCR. Applications include loss-of-function studies of iron uptake, cellular proliferation, ferroptosis, and tumor cell metabolism. Particles are ultra-purified and concentrated to high titer by PEG precipitation and sucrose gradient centrifugation, supporting efficient transduction of difficult-to-transfect cells, including primary and thawed cultures.
About This Product
This validated shRNA lentivirus targeting Tfrc (NCBI Accession: NM_011638) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, RFP, GFP/Luc, RFP/Luc) and antibiotic selection marker (Puromycin, Blasticidin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.
Knockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
