| Field | Specification |
|---|---|
| Target | |
| Alternative names | MIG12; TB10; migration-inducing gene 12|migration-inducing protein 12|thymosin; beta 10 |
| UniProt # | |
| Species | |
| Applications | |
| Assay type | |
| Sensitivity | |
| Detection range | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Thymosin-beta-10 is closely related in sequence to TMSB4 and is also an actin-sequestering protein. In most tissues of the rat, the same molecular weight species of TMSB10 mRNA is found; This finding, together with primer extension experiments, suggested that the 2 mRNAs are transcribed from the same gene through a combination of differential promoter utilization and alternative splicing. Both mRNAs were present in pachytene spermatocytes; only the testis-specific mRNA was detected in postmeiotic haploid spermatids. Immunohistochemical analysis showed that the protein was present in differentiating spermatids, suggesting that the testis-specific TMSB10 mRNA is translated in haploid male germ cells. thymosin-beta-10 protein synthesized in adult testis was identical in size to that synthesized in brain.
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate TMSB10 in samples. An antibody specific for TMSB10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMSB10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMSB10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMSB10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | 0.156-10 ng/mL |
| Sensitivity | 0.078 ng/mL |
| Species Reactivity | Mouse (Mus musculus) |
| Stability | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C). |
| Manufacturer No. | AE14102MO |
| UniProt # | Q6ZWY8 |
| Alternative Names | MIG12; TB10; migration-inducing gene 12|migration-inducing protein 12|thymosin; beta 10 |
Assay Workflow
The kit includes the components required for standard-curve quantification (see the kit manual for the component table and validated sample types). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.
Safety & Handling
For research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional Mouse ELISA kits from Wuhan Abebio Science Co Ltd, or contact us for related assays for TMSB10 research.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
