| Field | Specification |
|---|---|
| Target | |
| Alternative names | Metalloproteinase inhibitor 1;Collagenase inhibitor 16C8 fibroblast;Erythroid-potentiating activity;EPA;TPA-S1;TPA-induced protein;Tissue inhibitor of metalloproteinases 1;TIMP-1;Timp1;Timp, Timp-1; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: C25-R205 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse metalloproteinase inhibitor 1 (gene Timp1) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
TIMP-1 is a secreted tissue inhibitor of metalloproteinases that binds the catalytic zinc site of many matrix metalloproteinases, including MMP-9, and blocks their activity. It also has growth factor–like, MMP-independent effects on cell survival and proliferation. TIMP-1 is studied in extracellular matrix remodeling, fibrosis, inflammation and cancer.
Kit Components
| Anti-Mouse Timp1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse Timp1 Standard | 2 vials, 10 ng/tube |
| Mouse Timp1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 31.2 pg/mL–2,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant mouse Timp1 (Cys25–Arg205), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 5.3–6.4% (3 samples, n = 16 each) |
| Inter-assay CV | 6.4–8.3% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:5. In the supplier’s tests, serum and plasma samples measured 1024–4380 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.04; 31.2 = 0.13; 62.5 = 0.208; 125 = 0.364; 250 = 0.614; 500 = 1.002; 1000 = 1.59; 2000 = 2.232. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.04 | 0.13 | 0.208 | 0.364 | 0.614 | 1.002 | 1.59 | 2.232 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 65 | 258 | 787 | 62 | 246 | 710 |
| Standard deviation | 3.44 | 16.51 | 41.71 | 4.21 | 20.41 | 45.44 |
| CV (%) | 5.3% | 6.4% | 5.3% | 6.8% | 8.3% | 6.4% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Zhifu Xie et al. (2025) The enedioic acid analog 326E alleviates metabolic dysfunction-associated steatohepatitis via dual targeting at ACLY and PPARα. Cell Metabolism. 10.1016/j.cmet.2025.09.011
Jinhua Chi et al. (2019) Synthesis and anti-metastasis activities of norcantharidin-conjugated carboxymethyl chitosan as a novel drug delivery system. Carbohydrate Polymers. 10.1016/j.carbpol.2019.03.026
Zhiwen Jiang et al. (2017) Preparation and pharmacological evaluation of norcantharidin-conjugated carboxymethyl chitosan in mice bearing hepatocellular carcinoma. Carbohydrate Polymers. 10.1016/j.carbpol.2017.06.072
Zhiwen Jiang et al. (2015) Carboxymethyl chitosan represses tumor angiogenesis in vitro and in vivo. Carbohydrate Polymers. 10.1016/j.carbpol.2015.04.040
Erdoğan Mümin Alper et al. (2026) Tamoxifen as a Therapeutic Intervention for Neurobehavioral Deficits in a Propionic Acid-Induced Autism Model via Anti-inflammatory Mechanisms. Neurochemical Research. 10.1007/s11064-026-04730-w
T. T. Liao et al. (2017) Evaluation of the Size-Dependent Cytotoxicity of DLC (Diamondlike Carbon) Wear Debris in Arthroplasty Applications. ACS Biomaterials Science & Engineering. 10.1021/acsbiomaterials.6b00618
Te-Hua Liu et al. (2019) Isolation and identification of anti-periodontitis ingredients in Lactobacillus paracasei subsp. paracasei NTU 101-fermented skim milk in vitro. Journal of Functional Foods. 10.1016/j.jff.2019.103449
Chia-Yuan Chang et al. (2019) Lactobacillus paracasei subsp. paracasei NTU 101-fermented skim milk as an adjuvant to uracil-tegafur reduces tumor growth and improves chemotherapy side effects in an orthotopic mouse model of colorectal cancer. Journal of Functional Foods. 10.1016/j.jff.2019.02.025
Zhiwen Jiang et al. (2017) Evaluation on biological compatibility of carboxymethyl chitosan as biomaterials for antitumor drug delivery. Journal of Biomaterials Applications. 10.1177/0885328216688337
Guo Yujie et al. (2014) IL-22-producing Th22 cells play a protective role in CVB3-induced chronic myocarditis and dilated cardiomyopathy by inhibiting myocardial fibrosis. Virology Journal. 10.1186/s12985-014-0230-z
