| Field | Specification |
|---|---|
| Target | |
| Alternative names | BDNF/NT-3 growth factors receptor;2.7.10.1;GP145-TrkB/GP95-TrkB;Trk-B;Neurotrophic tyrosine kinase receptor type 2;TrkB tyrosine kinase;Ntrk2;Trkb; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: C32-H429 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse BDNF/NT-3 growth factors receptor (gene Ntrk2) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
TrkB (NTRK2) is a receptor tyrosine kinase activated mainly by BDNF and neurotrophin-4. Its signaling supports neuron survival, differentiation and synapse formation and is central to activity-dependent synaptic plasticity, so TrkB is widely studied in learning and memory, depression, neurodegeneration and obesity.
Kit Components
| Anti-Mouse Ntrk2 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse Ntrk2 Standard | 2 vials, 10 ng/tube |
| Mouse Ntrk2 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 93.8 pg/mL–6,000 pg/mL |
| Sensitivity | <5 pg/mL |
| Standard | Recombinant mouse Ntrk2 (Cys32–His429), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 6.4–6.9% (3 samples, n = 16 each) |
| Inter-assay CV | 6.8–7.4% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured 2–5 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.032; 93.8 = 0.097; 187.5 = 0.154; 375 = 0.235; 750 = 0.409; 1500 = 0.722; 3000 = 1.329; 6000 = 2.243. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.032 | 0.097 | 0.154 | 0.235 | 0.409 | 0.722 | 1.329 | 2.243 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 188 | 679 | 2008 | 202 | 622 | 2201 |
| Standard deviation | 12.22 | 46.85 | 128.51 | 13.73 | 46.02 | 149.66 |
| CV (%) | 6.5% | 6.9% | 6.4% | 6.8% | 7.4% | 6.8% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Ikezu Seiko et al. (2020) Inhibition of colony stimulating factor 1 receptor corrects maternal inflammation-induced microglial and synaptic dysfunction and behavioral abnormalities. Molecular Psychiatry. 10.1038/s41380-020-0671-2
Hsiu-Chuan Lee et al. (2019) Degree of lipid saturation affects depressive-like behaviour and gut microbiota in mice. International Journal of Food Sciences and Nutrition. 10.1080/09637486.2019.1681380
Zhijun Lu et al. (2018) Sevoflurane-induced memory impairment in the postnatal developing mouse brain. Experimental and Therapeutic Medicine. 10.3892/etm.2018.5950
