| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Product Type | |
| Sample Type(s) | cell culture supernatant, serum, plasma, tissue |
| Species | |
| Target |
Assay Principle
The TribioScience FAST Mouse Tyrosine Hydroxylase/TH ELISA is a solid-phase ELISA designed to measure mouse TH levels in cell culture supernatants, serum, plasma, and tissues. The kit uses our novel proprietary ELISA technology to combine sample and detection into a one-step, making the assay simple, easy, accurate, and fast. The entire procedure can be finished in 2 hours, not 5-6 hours.
Mouse Tyrosine Hydroxylase/TH ELISA Kit is a Solid-phase ELISA for the quantitative determination of Tyrosine Hydroxylase/TH. The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | Tyrosine Hydroxylase/TH |
|---|---|
| Species | Mouse |
| Sample Type | cell culture supernatant, serum, plasma, tissue |
| Test Principle | Solid-phase ELISA |
| Assay Time | 2 hours |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This kit is validated for cell culture supernatant, serum, plasma, tissue. Other matrices may require additional in-lab validation.
The total assay time is approximately 2 hours from sample addition to plate reading.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this sandwich ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
