| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vascular cell adhesion protein 1;V-CAM 1;VCAM-1;CD106;Vcam1;Vcam-1; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: F25-E698 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse vascular cell adhesion protein 1 (gene Vcam1) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
VCAM-1 (CD106) is an immunoglobulin-superfamily adhesion molecule expressed on activated endothelial cells. Induced by inflammatory cytokines, it binds the integrin VLA-4 (α4β1) on leukocytes and supports their firm adhesion and migration into tissues. VCAM-1 is a common marker of endothelial activation in inflammation and atherosclerosis research.
Kit Components
| Anti-Mouse Vcam1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse Vcam1 Standard | 2 vials, 10 ng/tube |
| Mouse Vcam1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 156 pg/mL–10,000 pg/mL |
| Sensitivity | <5 pg/mL |
| Standard | Recombinant mouse Vcam1 (Phe25–Glu698), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 6.8–7.2% (3 samples, n = 16 each) |
| Inter-assay CV | 7.7–8.7% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:500. In the supplier’s tests, serum and plasma samples measured 960–1200 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.001; 156 = 0.082; 312 = 0.174; 625 = 0.333; 1250 = 0.609; 2500 = 0.997; 5000 = 1.508; 10000 = 2.112. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.001 | 0.082 | 0.174 | 0.333 | 0.609 | 0.997 | 1.508 | 2.112 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 371 | 1198 | 4387 | 354 | 1298 | 4119 |
| Standard deviation | 26.8%2 | 86.25 | 298.31 | 29.02 | 112.92 | 317.16 |
| CV (%) | 6.8% | 7.2% | 6.8% | 8.2% | 8.7% | 7.7% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Xue-Xue Zhu et al. (2025) Sodium pump subunit NKAα1 protects against diabetic endothelial dysfunction by inhibiting ferroptosis through the autophagy-lysosome degradation of ACSL4. Clinical and Translational Medicine. 10.1002/ctm2.70221
Mingwei Xu et al. (2022) Genetic Arg-304-His substitution in GRK5 protects against sepsis progression by alleviating NF-κB-mediated inflammation. International Immunopharmacology. 10.1016/j.intimp.2022.109629
Li Chenglong et al. (2022) sVCAM1 in the Hippocampus Contributes to Postoperative Cognitive Dysfunction in Mice by Inducing Microglial Activation Through the VLA-4 Receptor. Molecular Neurobiology. 10.1007/s12035-022-02924-1
Dan-Ni Fu et al. (2021) Silenced long non-coding RNA activated by DNA damage elevates microRNA-495-3p to suppress atherosclerotic plaque formation via reducing Krüppel-like factor 5. Experimental Cell Research. 10.1016/j.yexcr.2021.112519
Mengling Chang et al. (2023) GEF-H1/RhoA signaling pathway mediates pro-inflammatory effects of NF-κB on CD40L-induced pulmonary endothelial cells. Molecular Immunology. 10.1016/j.molimm.2023.03.015
Yingying Lin et al. (2024) MiR-145 Alleviates Sepsis-Induced Inflammatory Responses and Organ Injury by Targeting ADAM17. Frontiers in Bioscience-Landmark. 10.31083/j.fbl2901044
Xiao-li Wang et al. (2019) The role of PSGL-1 in pathogenesis of systemic inflammatory response and coagulopathy in endotoxemic mice. Thrombosis Research. 10.1016/j.thromres.2019.08.019
Yuzhu Zhang et al. (2016) Protective Effect of Irisin on Atherosclerosis via Suppressing Oxidized Low Density Lipoprotein Induced Vascular Inflammation and Endothelial Dysfunction. PLoS One. 10.1371/journal.pone.0158038
