| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
mRNA Cap 2´-O-Methyltransferase is a recombinant protein derived from vaccinia virus. The enzyme can add a methyl group at the 2´-O position of the first nucleotide of the cap structure at the 5´ end of the RNA. The enzyme uses SAM as a methyl donor to methylate capped RNA to form a Cap1 structure. The Cap1 structure can enhance the translation efficiency of mRNA, so it can improve the expression of mRNA in transfection and microinjection experiments. This enzyme requires RNA with m7GpppN, a 7-methylguanosine cap structure, as a substrate.
This product is produced in accordance with GMP process requirements. The product is provided in liquid form and can be used for Cap1 capping reaction of pre-mRNA in vivo/in vitro.
Feature
- Isolated from a recombinant source
- Tested for the absence of endonucleases, exonucleases, Rnases
Application
- 2´-O-methylation of capped in vitro transcripts helps the RNA evade innate immune response in vivo
- mRNA cap-1 structure may improve expression
Specification
| Source | Recombinant E.coli with Cap 2´-O-Methyltransferase gene |
| Optimum Temperature | 37℃ |
| Storage Buffer | 20 mM Tris-HCl pH8.0, 0.1mM EDTA, 1mM DTT, 100mM NaCl, 50% (v/v) glycerin, 0.1% (v/v) Trion X-100 |
| Unit Definition | One unit is defined as the amount of enzyme required to methylate 10 pmol of 80nt capped RNA transcript in 1 h at 37°C. |
Components
| Components No. | Name | 10612ES92 (10,000 U) | 10612ES97 (50,000 U) | 10612ES98 (250,000 U) | 10612ES99 (20 MU) |
| 10612 | mRNA Cap 2´-O-Methyltransferase GMP-grade (50 U/μl) | 200 μL | 1 mL | 5 mL | 400 mL |
Shipping and Storage
The mRNA Cap 2´-O-Methyltransferase GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
Figures
Figures 1. The relationship between capping efficiency and 2’-O-Methyltransferase quantity
Glycerol Content: Contains Glycerol
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
This enzyme is validated for use in In Vitro Transcription applications. It is suitable for use in both standard laboratory research settings and, where applicable, optimized reaction workflows requiring high specificity, sensitivity, or throughput.
Capping efficiency (%) is determined by the proportion of RNA transcripts bearing a 5′ cap structure following the standard capping reaction. Activity is also expressed as units (U) per µL under manufacturer-defined assay conditions.
This enzyme is produced as Recombinant (E. coli) and is available in GMP grade for regulated applications. This enzyme is manufactured in an ISO 13485-certified UCF.ME™ facility and meets GMP-grade quality standards. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
A 5′ cap structure (typically 7-methylguanosine, m7G) on mRNA is essential for ribosomal recognition during cap-dependent translation initiation via eIF4E binding. It also protects mRNA from 5′ exonuclease degradation and signals to the innate immune system that the transcript is "self." Cap-1 structures (2′-O-methylation at the +1 nucleotide) further reduce immunogenicity, which is critical for therapeutic mRNA vaccines and protein replacement therapies.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
