| Field | Specification |
|---|---|
| Mfr No | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Recombinant human MSP1D1 (membrane scaffold protein 1D1) encompassing amino acids 79-267 (end). In this construct the N-terminal end of the protein is fused to a His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag (TEV: tobacco etch virus cysteine protease (BPS Bioscience #50308)). The protein was affinity purified.
Product Description
Recombinant ProteinRecombinant MSP1D1 Human protein is produced using a validated recombinant expression system and supplied in aqueous buffer solution. Suitable for enzyme kinetics, inhibitor screening, binding assays, structural studies, and related biochemical research applications.
Protein Specifications
| UniProt ID | P02647 |
|---|---|
| Amino Acids / Region | 79-267(end) |
| Molecular Weight | 25 kDa |
| Purity | ≥90% |
| Formulation | 20 mM Tris-HCl, pH 7.4, 100 mM NaCl, 0.5 mM EDTA |
| Storage | Aqueous buffer solution. |
| Biosafety Level | Not applicable (BSL-1) |
Safety & Handling
Avoid freeze/thaw cycles.
This protein was produced in a recombinant expression system. Expression system selection determines post-translational processing, disulfide bond formation, and co-factor incorporation — all of which affect enzymatic activity. Insect cell (Sf9) systems are preferred for kinases and multi-subunit enzymes that require phosphorylation or chaperone assistance; E. coli is used for structurally simpler proteins.
This protein spans amino acids 79-267(end). Confirm the region includes your domain of interest — the active site, binding pocket, or substrate recognition sequence — before placing your order. Refer to the UniProt database for domain annotation.
≥90% as determined by SDS-PAGE. A gel image is provided with each lot. BPS Bioscience performs rigorous QC on each lot, including purity assessment and functional activity testing where applicable. Contact technical support if purity ≥99% is required for biophysical measurements.
This protein is suitable for biochemical assays including enzyme kinetics, inhibitor screening, protein binding studies, and drug discovery. Refer to the product datasheet for validated protocols and recommended assay conditions. Contact BioHippo technical support for application-specific guidance.
Aqueous buffer solution. Avoid repeated freeze-thaw cycles — prepare single-use working aliquots. Add BSA or glycerol to aliquots if storing diluted enzyme is necessary. Typical stability is at least 6 months at −80°C.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Bulk quantities: Large-scale orders for HTS campaigns or structural studies.
- Custom constructs: Alternative tag positions, truncation variants, or point mutants may be available upon request.
- Biotinylated variants: Avi-Tag site-specific biotinylation is available for SPR/BLI surface capture applications.
- Extended QC data: Activity assay data, SEC-HPLC profiles, or additional purity methods available on request.
Contact BioHippo customer support for custom requirements.
