| Field | Specification |
|---|---|
| Mfr No | |
| Species |
The MSTO-211H cell line is derived from a patient with biphasic mesothelioma, specifically from a pleural effusion. It is classified as metastatic, and the patient had not undergone prior radiation or chemotherapy treatments before the establishment of the cell line. MSTO-211H cells are notable for expressing several markers that are significant in understanding both their biological behavior and their potential utility in cancer research. These cells possess high-affinity binding sites for epidermal growth factor (EGF), a property that may contribute to their proliferative capabilities, as EGF is a key regulator of cell growth and differentiation. The presence of EGF receptors suggests these cells could be useful in studying pathways related to growth factor signaling in cancer. In addition to EGF receptors, MSTO-211H cells express neuron-specific enolase (NSE), an enzyme typically found in neurons and neuroendocrine cells. NSE expression in MSTO-211H cells may be indicative of a neuroendocrine differentiation potential, a feature that can be significant for understanding the heterogeneity of mesothelioma tumors. Furthermore, the cells express both the alpha and beta subunits of human chorionic gonadotropin (HCG), a hormone typically produced during pregnancy but also known to be secreted by certain cancers. The expression of HCG subunits in MSTO-211H cells suggests a possible role in tumor biology, potentially related to immune evasion or tumor progression mechanisms. These markers collectively highlight the complex nature of this cell line, making it a valuable model for investigating mesothelioma biology and the effects of therapeutic agents.
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- Protein expression: High affinity binding sites for EGF, expression of Neuron specific enolase (NSE) and alpha and beta subunits of HCG, L-DOPA decarboxylase (DDC), bombesin and neurotensin were not detected.
- Tumorigenic: Yes, tumors for med in approximately 20% of nude mice inoculated with MSTO-211H cells
- Karyotype: modal number = 72, range = 70 to 78
- cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- doublingTime: 20 hours
- subculturing: The cells can reach a saturation density of 400.000 cells per cm2, but will slough off the surface as they attain this density. Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
- seedingDensity: 1 x 104 cells/cm2
- fluidRenewal: 2 to 3 times per week
- postThawRecovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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