Mv.1.Lu cell

SKU:BHC11101269
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Overview
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Mv.1.Lu cell is a cell line (Mixed sex). It is commonly used as an in vitro model for 1 research. Growth characteristics: Adherent, Epithelial. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Neovison vison (American mink)
Morphology Epithelial
Growth Properties Adherent
Tissue Lung
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Catalog no. Size
305192 1 cryovial
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 305192
Species Neovison vison (American mink)
The Mv.1.Lu cell line, also known as CCL 64, originates from the lung tissue of a fetal mink (Mustela vison). It is an epithelial-like cell line known for its flat, contact-inhibited growth in monolayer cultures, exhibiting a regular polygonal morphology. This cell line has been widely utilized in virological studies due to its broad permissivity for various mammalian Type C viruses, including murine and feline sarcoma viruses, making it a preferred system for focus formation assays and viral transformation studies. This cell line's ability to support viral replication and transformation has made it an essential tool in understanding viral pathogenesis and host-pathogen interactions. Mv.1.Lu cells are also used in pulmonary physiology research, benefiting from their origin in lung tissue. Studies on their growth characteristics, including their response to various media and culture conditions, have underscored their adaptability and stability in laboratory settings.

SKU:BHC11101269

  • cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
  • supplements: Supplement the medium with 10% FBS and 1% NEAA
  • dissociationReagent: Accutase
  • subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
  • fluidRenewal: 2 to 3 times per week
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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