| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
MYB (c-MYB) is a transcription factor and the founding member of the MYB family, which also includes MYBL1 (A-MYB) and MYBL2 (B-MYB). MYB binds specific DNA elements to regulate genes that block differentiation and sustain proliferation in immature cells. It is essential for the maintenance and expansion of hematopoietic stem and progenitor cells and is expressed in colonic crypt and breast epithelial cells. Because MYB activity favors a continued proliferative, undifferentiated state, its deregulation contributes to the tumorigenesis of several cancers, particularly leukemia. MYB is therefore an important target for studies of hematopoiesis, differentiation, and oncogenic transcription.
Product Description & Applications
The MYB Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of MYB activity in human or mouse cells. The construct uses tandem repeats of the consensus MYB DNA-binding element, a core sequence shared with MYBL1, to detect MYB transcriptional activity and the signaling pathways that activate it. A range of reporters (GFP, RFP, EGFP, d2GFP, mCherry, firefly luciferase) and antibiotic selection markers (puromycin or blasticidin) support tracking of transduced cells and establishment of stable reporter cell lines. The system is applied to studies of hematopoietic proliferation, differentiation, and leukemogenesis. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.
About This Product
This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, Luc, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the MYB transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
