| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, N/A, Puromycin |
| Shipping | |
| Species |
Background
MYC is a basic helix-loop-helix leucine zipper transcription factor and a central regulator of cell growth, proliferation, metabolism, ribosome biogenesis, and apoptosis. By dimerizing with its partner MAX and binding E-box elements, MYC activates or represses thousands of target genes that coordinate the cell cycle and biosynthetic capacity. MYC protein levels are tightly controlled by phosphorylation-dependent degradation; phosphorylation at threonine 58 promotes its turnover. The T58A point mutation abolishes this signal, stabilizing the protein. MYC is one of the most frequently deregulated oncogenes in human cancer, where its overexpression or stabilization drives uncontrolled proliferation, making it a major focus of oncology research.
Product Description & Applications
The MYC (Wild Type & Mutant) ORF cDNA Lentivirus enables stable overexpression of the human or mouse MYC open reading frame in mammalian cells, available as wild-type and the stabilizing T58A mutant. A CMV or EF1a promoter drives the MYC ORF fused to a C-terminal V5 tag, with a fluorescent reporter (GFP or RFP) and antibiotic selection marker separated by a self-cleaving peptide for independent translation from a single transcript. The optimized third-generation vector provides high expression and reliable genome integration for rapid stable cell line generation. Applications include studying MYC-driven proliferation, metabolism, transformation, and the functional impact of T58A-mediated protein stabilization. Particles transduce difficult-to-transfect cells, including primary and thawed cells; cloning is sequence-verified and expression validated by transient transfection.
About This Product
This ORF cDNA lentivirus enables stable overexpression of MYC (NCBI Accession: NM_002467, NM_010849) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (GFP, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.
Ultra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
