| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | PDGF-BB; Platelet-derived growth factor subunit B; c-sis proto-oncogene product |
| Biological Activity | |
| Endotoxin Level | |
| Expression System | |
| Form | Lyophilized powder |
| Formulation | |
| Gene ID | |
| Molecular Weight | |
| Post-Translational Modifications | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage | |
| Target |
PDGF-BB (Platelet-Derived Growth Factor BB) is a potent mitogen with pleiotropic roles in mesenchymal and stromal cell biology. It signals through PDGFRα and PDGFRβ receptor tyrosine kinases, activating RAS/MAPK, PI3K/Akt, and PLCγ/PKC cascades to drive cell proliferation, migration, and survival. Uniquely among PDGF isoforms, PDGF-BB binds and activates all three receptor combinations — PDGFRαα, PDGFRαβ, and PDGFRββ — enabling broad targeting across connective tissue, vascular, and progenitor cell populations.
Expressed in Pichia pastoris (K. phaffii)
MycoFold™ PDGF-BB is produced in a proprietary engineered yeast platform that ensures chaperone-guided protein folding, lysis-free secretion, and elimination of animal-origin inputs and bacterial endotoxin. The result is a highly pure, bioactive protein that reconstitutes in deionized water — no special buffers required.
Product Specifications
| Cat. No. | GR1002-10, GR1002-50, GR1002-100, GR1002-250, GR1002-500, GR1002-1000 |
|---|---|
| Source | Pichia pastoris (K. phaffii) |
| Structure / Form | Carrier protein-free; no affinity tags; lyophilized with no excipients |
| Molecular Weight | ~28 kDa (homodimer, non-reduced); ~14 kDa (monomer, reduced) |
| UniProt / Gene | P01127 / PDGFB (NCBI Gene ID: 5155) |
| Purity | >95% by SDS-PAGE and HP-SEC |
| Endotoxin | <0.1 EU/µg by LAL |
| Storage | Lyophilized: ≤−20°C until expiry. Reconstituted: 2–8°C up to 2 weeks; −20°C for extended storage. Avoid repeated freeze-thaw cycles. |
| Shipping | Ambient temperature; lyophilized formulation requires no cold chain |
QC & Validation
✓ Lot-specific CoA ✓ SDS-PAGE Purity ✓ LAL Endotoxin Tested ✓ Mycoplasma Tested (qPCR) ✓ BSE/TSE Statement AvailableSafety & Regulatory
| Cat. No. | GR1002-10, GR1002-50, GR1002-100, GR1002-250, GR1002-500, GR1002-1000 |
| Expression System | Pichia pastoris (K. phaffii), eukaryotic yeast |
| Protein Structure | Disulfide-linked B:B homodimer; lyophilized powder |
| Molecular Weight | ~28 kDa (homodimer, non-reduced); ~14 kDa (monomer, reduced) |
| UniProt / Gene | P01127 / PDGFB (NCBI Gene ID: 5155) |
| Species Reactivity | Human |
| EC50 (Activity) | 7.2–8.2 ng/mL (M-NFS-60 cells, 72-hr Prestoblue™ proliferation assay) |
| Specific Activity | ≥1.36×10⁵ IU/mg (>4.4× leading E. coli competitor) |
| Bioactivity Value | 43.6 IU/mg/$ (>6.3× E. coli competitor) |
| Purity | >95% by SDS-PAGE and HP-SEC |
| Endotoxin | <0.1 EU/µg by LAL |
| Thermal Stability (Tm) | 43°C (Biotium GloMelt™ Thermal Shift assay, triplicate) |
| Formulation | Carrier protein-free; no affinity tags; no excipients added |
| Reconstitution | Deionized water, 0.1–0.5 mg/mL; no buffers required |
| Storage (Lyophilized) | ≤−20°C until expiry |
| Storage (Reconstituted) | 2–8°C up to 2 weeks; −20°C for extended storage; avoid repeated freeze-thaw |
| Shipping | Ambient temperature; no cold chain required |
Bioactivity Assay: Dose-dependent stimulation of M-NFS-60 cell proliferation (ATCC Cat. No. 30-2001) over 72 hr was assessed by Prestoblue™ Cell Viability luminescence. MycoFold rHu PDGF-BB achieved an EC50 of 7.2–8.2 ng/mL, with specific activity of ≥1.36×10⁵ IU/mg — over 4.4× higher than the leading E. coli-expressed competitor (3.10×10⁴ IU/mg) and >6.3× greater bioactivity per research dollar.
Thermal Stability: Melting temperature (Tm) was determined in triplicate using the Biotium GloMelt™ Thermal Shift Protein Stability Kit (Cat. No. 33022-T), measuring relative fluorescence by RT-PCR across 30–100°C. MycoFold rHu PDGF-BB Tm = 43°C (vs. Goat IgG control Tm = 72.6°C). Native disulfide bridges and proper 3D folding maintain bioactivity after reconstitution and following refrigerated storage or a single freeze-thaw cycle.
Stability Testing: Natively folded MycoFold rHu PDGF-BB maintains its high bioactivity after 1× freeze-thaw and after refrigerated storage (verified by lot-specific CoA specific activity data).
MycoFold™ rHu PDGF-BB supports research across oncology, neuroscience, vascular biology, and regenerative medicine:
- Oncology / Tumor Stroma: Cancer-associated fibroblast (CAF) activation, tumor stroma formation, autocrine/paracrine tumor progression models
- Neurological & Vascular: Blood-brain barrier integrity studies, oligodendrocyte precursor cell (OPC) differentiation & remyelination, angiogenesis & vascular stabilization assays
- Regenerative & Tissue Repair: Wound healing models, musculoskeletal repair, bone and cartilage differentiation, hydrogel and scaffold-based platforms
- Progenitor & Stem Cells: Mesenchymal stem cell (MSC) stimulation, osteoblast/chondrocyte differentiation, pericyte recruitment assays
- Receptor Signaling: RAS/MAPK (MEK/ERK), PI3K/Akt, and PLCγ/PKC pathway activation studies across all three PDGFR receptor combinations (PDGFRαα, PDGFRαβ, PDGFRββ)
MycoFold rHu PDGF-BB is expressed in a proprietary engineered Pichia pastoris (Komagataella phaffii) yeast system. Unlike E. coli, this eukaryotic system supports chaperone-guided protein folding in the ER and lysis-free secretion, enabling proper disulfide bridge formation and native-like 3D structure without animal-origin inputs or bacterial endotoxin contamination.
In head-to-head testing against the leading E. coli-expressed PDGF-BB, MycoFold achieved >4.4× higher specific activity (1.36×10⁵ vs 3.10×10⁴ IU/mg) and >6.3× greater bioactivity value per research dollar (43.6 vs 7.0 IU/mg/$). The superior activity stems from precise disulfide bridge formation and correct receptor-binding residue orientation, which are difficult to achieve in bacterial systems.
Reconstitute lyophilized rHu PDGF-BB in deionized water to 0.1–0.5 mg/mL. No special buffers, carrier proteins, or supplements are required. Allow the vial to equilibrate to room temperature before opening. After reconstitution, store at 2–8°C for up to 2 weeks, or at −20°C for extended storage. Avoid repeated freeze-thaw cycles to preserve bioactivity.
PDGF-BB is unique among PDGF isoforms in its ability to bind and activate all three receptor dimeric combinations: PDGFRαα, PDGFRαβ, and PDGFRββ. This broad receptor binding profile enables activation of downstream RAS/MAPK (MEK/ERK), PI3K/Akt, and PLCγ/PKC signaling cascades in connective tissue, vascular, and progenitor cell populations.
Yes. Every lot of MycoFold rHu PDGF-BB is released with a lot-specific Certificate of Analysis (CoA) documenting activity testing (EC50, specific activity), molecular mass (SDS-PAGE), purity (>95% by HP-SEC), endotoxin (<0.1 EU/µg by LAL), and mycoplasma testing. A BSE/TSE statement and Material Safety Data Sheet (MSDS) are also available upon request.
Need a tailored solution for your research? The following customization options may be available for qualified research programs:
- Custom Lot Sizes: Quantities >1 mg available for high-throughput or longitudinal studies — contact us for volume pricing
- Bulk/Custom Pricing: Volume discount programs available for core labs and institutional procurement partners
- GMP-Grade Supply: Scalable to GMP manufacturing for preclinical and cell therapy process development applications
- Early Evaluation Sample: Qualified researchers may request a complimentary 10 µg evaluation sample to benchmark against your current supplier
Contact our scientific support team to discuss your specific requirements.
